Allameh Maryam, Khalesi Saeedeh, Khozeimeh Faezeh, Faghihian Elham
Dental Research Center, Department of Oral Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Dental Material Research Center, Department of Oral and Maxillofacial Pathology, Isfahan University of Medical Sciences, Isfahan, Iran.
J Lasers Med Sci. 2018 Summer;9(3):194-199. doi: 10.15171/jlms.2018.35. Epub 2018 Jul 28.
The aim of the present study was to compare the effects of laser and basic fibroblastic growth factor (bFGF) treatment on operative wound healing in a rat model. Sixty-six male Wistar rats were employed in this study. A 10-mm surgical wound was created on the buccal mucosa of each rat, under anesthesia, and then the rats were divided into 3 groups of 22: (1) GF group (received subcutaneous injection of bFGF), (2) laser group (treated with low-level laser irradiation), and (3) control group (received no treatment). On day 5, half of the rats in each group and on day 10 the other half, were sacrificed. Afterward, samples were taken from rats' buccal mucosa for histological assay and scoring. The data were analyzed using MannWhitney test (α =5%). On day 5 there was not any significant difference between GF and control groups; however, the laser group showed clinically delayed wound coverage, compared to other groups (<0.05). On day 10, histological examination demonstrated marked vascular granulation tissue ( GT) in GF group. Collagen production was significantly prominent in laser group compared to GF treated samples (=0.004). Inflammation of GT in GF and laser groups was significantly less than that in control samples (=0.005 and =0.001, respectively). The components of wound matrix induced by GF and laser treatment were significantly different. Although bFGF or laser treatment of oral wounds, under the conditions of the present study, did not accelerate wound healing, they showed some other notable effects on the quality of healing.
本研究的目的是比较激光和碱性成纤维细胞生长因子（bFGF）治疗对大鼠模型手术伤口愈合的影响。本研究采用了66只雄性Wistar大鼠。在麻醉状态下，于每只大鼠的颊黏膜上制造一个10毫米的手术伤口，然后将大鼠分为3组，每组22只：（1）GF组（接受皮下注射bFGF），（2）激光组（接受低强度激光照射治疗），以及（3）对照组（未接受治疗）。在第5天，每组一半的大鼠被处死，另一半在第10天被处死。之后，从大鼠的颊黏膜取样进行组织学分析和评分。数据采用Mann-Whitney检验进行分析（α = 5%）。在第5天，GF组和对照组之间没有任何显著差异；然而，与其他组相比，激光组在临床上显示伤口覆盖延迟（<0.05）。在第10天，组织学检查显示GF组有明显的血管肉芽组织（GT）。与GF治疗的样本相比，激光组的胶原蛋白生成明显更显著（=0.004）。GF组和激光组GT的炎症明显少于对照样本（分别为=0.005和=0.001）。GF和激光治疗诱导的伤口基质成分有显著差异。尽管在本研究条件下，bFGF或激光治疗口腔伤口并未加速伤口愈合，但它们对愈合质量显示出一些其他显著影响。
