Du Zhaokui, Lin Weida, Yu Binbin, Zhu Jinxing, Li Junmin
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, China.
Taizhou Vocational College of Science and Technology, Taizhou, China.
Front Plant Sci. 2022 Feb 8;12:794137. doi: 10.3389/fpls.2021.794137. eCollection 2021.
is a medicinal plant containing flavonoids, triterpenoids, polyphenolics, polysaccharides, and other compounds with diverse biological functions. is distributed across altitudes ranging from 400 to 1,000 m. However, little is known about the effect of altitude on metabolite accumulation in Also, the biosynthetic pathway involved in flavonoid accumulation in has not been fully elucidated. In this study, mature leaves of growing at low altitude (280 m) and high altitude (920 m) were sampled and subjected to metabolomic and transcriptomic analyses. The flavonoid content and composition were higher in the leaves of collected at high altitude than in those collected at low altitude. Most of the differentially accumulated metabolites (DAMs) were enriched in "flavone and flavonol biosynthesis." The significant differentially expressed genes (DEGs) between low and high altitudes were mainly enriched in "biological process." The most heavily enriched KEGG pathway was related to the subcategory "Oxidative phosphorylation," indicating that complicated biological processes are involved in the response of to harsh environmental factors. High UV-light might be the main influencing factor among the harsh environmental factors found in high altitudes. Integrated analysis of metabolomic and transcriptomic data showed that 31 flavonoids were significantly correlated with 227 DEGs, resulting in 412 related pairs (283 positive and 129 negative) between the DEGs and flavonoids. The possible mechanisms underlying the differentially accumulation of flavonoids at different altitude might be due to variations in transport and relocation of flavonoids in leaves, but not different flavonoid biosynthesis pathways. The up-regulation of genes related to energy and protein synthesis might contribute to flavonoid accumulation at high altitudes. This study broadens our understanding of the effect of altitude on metabolite accumulation and biosynthesis in
是一种含有黄酮类化合物、三萜类化合物、多酚类化合物、多糖及其他具有多种生物学功能化合物的药用植物。它分布于海拔400至1000米的区域。然而，关于海拔对其代谢物积累的影响知之甚少。此外，其黄酮类化合物积累所涉及的生物合成途径尚未完全阐明。在本研究中，采集了生长在低海拔（280米）和高海拔（920米）的成熟叶片，并进行了代谢组学和转录组学分析。高海拔采集的叶片中黄酮类化合物的含量和组成高于低海拔采集的叶片。大多数差异积累代谢物（DAMs）富集于“黄酮和黄酮醇生物合成”。低海拔和高海拔之间显著差异表达的基因（DEGs）主要富集于“生物学过程”。富集程度最高的KEGG途径与“氧化磷酸化”亚类相关，表明其对恶劣环境因素的响应涉及复杂的生物学过程。高海拔发现的恶劣环境因素中，高紫外线可能是主要影响因素。代谢组学和转录组学数据的综合分析表明，31种黄酮类化合物与227个DEGs显著相关，导致DEGs与黄酮类化合物之间有412个相关对（283个正相关和129个负相关）。不同海拔黄酮类化合物差异积累的潜在机制可能是由于其叶片中黄酮类化合物转运和重新定位的差异，而非黄酮类化合物生物合成途径不同。与能量和蛋白质合成相关基因的上调可能有助于高海拔地区黄酮类化合物的积累。本研究拓宽了我们对海拔对其代谢物积累和生物合成影响的理解
