Department of Chemistry and Materials Engineering, Chung Cheng Institute of Technology, National Defense University, Tashi, Taoyuan, 33551, Taiwan.
Department of Chemistry and Materials Engineering, Chung Cheng Institute of Technology, National Defense University, Tashi, Taoyuan, 33551, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2019 May;98:177-184. doi: 10.1016/j.msec.2018.12.142. Epub 2019 Jan 2.
Organic-inorganic hybrid antibacterial materials based on Ag/AgCl and quaternary ammonium-modified silicate (Ormosil(NRCl)) were prepared by sol-gel processes and an in situ reduction method. The physical properties of Ormosil(NRCl) and the Ormosil(NRCl)/Ag hybrids were examined using FTIR, UV-Vis, SEM, XRD and NMR spectroscopy. The results indicated that Ag/AgCl was incorporated into the Ormosil(NRCl) matrix after impregnation. Morphological analysis by SEM showed uniformly-distributed Ag and AgCl particles in the Ormosil(NRCl) matrix, of spherical nature and a size around 5-20 and <200 nm. The antibacterial effects of Ormosil(NRCl) and the Ormosil(NRCl)/Ag hybrids against Gram-negative P. aeruginosa and E. coli, and Gram-positive S. aureus and B. subtilis, were assessed in terms of the zone of inhibition, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and plate-counting method, and an excellent antibacterial performance was discovered. Moreover, the antibacterial performance of the Ormosil(NRCl)/Ag hybrid was better than that of Ormosil(NRCl) and the Ormosil/Ag hybrids.
采用溶胶-凝胶法和原位还原法制备了基于 Ag/AgCl 和季铵盐改性硅酸钠（Ormosil（NRCl））的有机-无机杂化抗菌材料。采用傅里叶变换红外光谱（FTIR）、紫外-可见光谱（UV-Vis）、扫描电子显微镜（SEM）、X 射线衍射（XRD）和核磁共振波谱（NMR）对 Ormosil（NRCl）和 Ormosil（NRCl）/Ag 杂化物的物理性质进行了研究。结果表明，Ag/AgCl 是在浸渍后被掺入到 Ormosil（NRCl）基体中的。SEM 形貌分析表明，Ag 和 AgCl 颗粒均匀分布在 Ormosil（NRCl）基体中，呈球形，粒径约为 5-20nm 和 <200nm。通过抑菌圈、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和平板计数法评估了 Ormosil（NRCl）和 Ormosil（NRCl）/Ag 杂化物对革兰氏阴性菌铜绿假单胞菌和大肠杆菌，以及革兰氏阳性菌金黄色葡萄球菌和枯草芽孢杆菌的抗菌性能，发现具有优异的抗菌性能。此外，Ormosil（NRCl）/Ag 杂化物的抗菌性能优于 Ormosil（NRCl）和 Ormosil/Ag 杂化物。
