Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, Binghamton University; Johnson City, NY 13790, USA.
Department of Health Outcomes and Administrative Sciences, School of Pharmacy and Pharmaceutical Sciences, Binghamton University; Johnson City, NY 13790, USA.
Molecules. 2019 Feb 22;24(4):801. doi: 10.3390/molecules24040801.
Lung cancer is the leading cause of cancer-related death in the Unites States, and approximately 85% of all lung cancers are classified as non-small cell lung cancer (NSCLC), which is extremely difficult to treat and its survival rate is low. After decades of clinical trials, the most effective treatments are still those that implement the first-generation platinum anticancer agent cisplatin (CDDP) in combination with other drugs. We previously demonstrated that the naturally-occurring compound phenethyl isothiocyanate (PEITC) can be used to sensitize NSCLC cells to CDDP. Furthermore, co-encapsulation of PEITC and CDDP in liposomes enhances their toxicity toward NSCLC cells. We here optimize liposomal-PEITC-CDDP, demonstrate the release of PEITC and CDDP from the nanoparticle, and show that liposomal-PEITC-CDDP is much more toxic toward both A549 and H596 human NSCLC cell lines than toward WI-38 and BEAS-2B human normal lung cell lines. Thus, we have prepared an efficacious therapy that has significantly higher toxicity toward cancer cell lines than normal cell lines.
肺癌是美国癌症相关死亡的主要原因，约 85%的肺癌被归类为非小细胞肺癌（NSCLC），这种癌症极难治疗，存活率低。经过几十年的临床试验，最有效的治疗方法仍然是第一代铂类抗癌药物顺铂（CDDP）与其他药物联合使用。我们之前的研究表明，天然存在的化合物苯乙基异硫氰酸酯（PEITC）可用于增强 NSCLC 细胞对 CDDP 的敏感性。此外，将 PEITC 和 CDDP 共包封在脂质体中可增强它们对 NSCLC 细胞的毒性。我们在此优化了脂质体-PEITC-CDDP，证明了 PEITC 和 CDDP 从纳米颗粒中的释放，并表明脂质体-PEITC-CDDP 对 A549 和 H596 人非小细胞肺癌细胞系的毒性比 WI-38 和 BEAS-2B 人正常肺细胞系高得多。因此，我们已经制备了一种有效的治疗方法，对癌细胞系的毒性明显高于正常细胞系。
