Blondel Delphine, Lutolf Matthias P
Chimia (Aarau). 2019 Feb 27;73(1-2):81-85. doi: 10.2533/chimia.2019.81.
Organoids have stepped into the limelight as unique systems for modeling organ development, function and disease. This review provides a perspective on how chemically defined, bio-inspired hydrogels could be used for replacing ill-defined matrices derived from the native extracellular matrix (ECM) that are used for generating organoids in 3D stem cell culture. In particular, we propose the use of self-healing and light-responsive matrices that should afford control over the inherently stochastic self-organization process that currently underlies organoid morphogenesis. Such designer ECMs could accelerate the translation of organoid technology from the laboratory into various real-life applications.
类器官作为用于模拟器官发育、功能和疾病的独特系统已成为焦点。本综述探讨了如何使用化学成分明确、受生物启发的水凝胶来替代源自天然细胞外基质（ECM）的成分不明确的基质，这些基质目前用于三维干细胞培养中生成类器官。特别是，我们提议使用自愈和光响应性基质，这应能控制目前作为类器官形态发生基础的内在随机自组织过程。这种经过设计的ECM可以加速类器官技术从实验室向各种实际应用的转化。
