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Enzyme Microb Technol. 2016 May;86:7-16. doi: 10.1016/j.enzmictec.2016.01.004. Epub 2016 Jan 15.
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Isoflavones: chemistry, analysis, functions and effects on health and cancer.异黄酮：化学、分析、功能以及对健康和癌症的影响
Asian Pac J Cancer Prev. 2014;15(17):7001-10. doi: 10.7314/apjcp.2014.15.17.7001.
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Substituent effects on in vitro antioxidizing properties, stability, and solubility in flavonoids.取代基对黄酮类化合物体外抗氧化性能、稳定性及溶解性的影响。
J Agric Food Chem. 2014 Apr 16;62(15):3321-33. doi: 10.1021/jf405570u. Epub 2014 Apr 4.
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Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300.利用来自地热能球菌 DSM 11300 的重组淀粉蔗糖酶生物合成（+）-儿茶素糖苷。
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利用异源表达的麦芽糖基转移酶对大豆苷进行酶促修饰 。 你提供的原文最后似乎不完整，我根据常见语境进行了补充翻译。如果实际情况并非如此，请你提供完整准确的内容。

Enzymatic modification of daidzin using heterologously expressed amylosucrase in .

作者信息

Kim Eun-Ryoung, Rha Chan-Su, Jung Young Sung, Choi Jung-Min, Kim Gi-Tae, Jung Dong-Hyun, Kim Tae-Jip, Seo Dong-Ho, Kim Dae-Ok, Park Cheon-Seok

机构信息

1Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104 Republic of Korea.

2School of Food and Animal Science, Chungbuk National University, Cheongju, 28644 Republic of Korea.

出版信息

Food Sci Biotechnol. 2018 Aug 18;28(1):165-174. doi: 10.1007/s10068-018-0453-7. eCollection 2019 Feb.

DOI:10.1007/s10068-018-0453-7
PMID:30815307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6365322/
Abstract

Amylosucrases (ASase, EC 2.4.1.4) from (DGAS) and (NPAS) were heterologously expressed in While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in . Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.

摘要

来自[具体来源1]的淀粉蔗糖酶（ASase，EC 2.4.1.4）（DGAS）和来自[具体来源2]的（NPAS）在[表达宿主1]中进行了异源表达。虽然DGAS成功表达，但NPAS未成功表达。相反，NPAS在[表达宿主2]中表达。重组DGAS和NPAS通过镍亲和色谱法进行纯化，并用于修饰大豆苷以提高其水溶性和生物利用度。LC/MS分析表明，使用DGAS进行转糖基化的主要产物是大豆苷元二糖苷和大豆苷元三糖苷，而NPAS得到的产物仅为大豆苷元二糖苷。在pH 7.0的磷酸钠中，预测在大豆苷衍生物中具有最高水溶性的大豆苷元三糖苷的最佳生物转化条件为4%（w/v）蔗糖和250 mg/L大豆苷，反应时间为12小时。综上所述，我们认为异黄酮大豆苷转糖基化的产率和产物特异性可能受ASase来源和反应条件的调节。