Bae Chun-Sik, Yun Chul-Ho, Ahn Taeho
1College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186 Republic of Korea.
2School of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea.
Food Sci Biotechnol. 2018 Aug 3;28(1):175-180. doi: 10.1007/s10068-018-0445-7. eCollection 2019 Feb.
In this study, it was demonstrated that 1,3-dichloro-2-propanol (1,3-DCP) induced oxidative stress and cell death in HuH7, human hepatocytes. The protective effects of () and Uyeki ( Uyeki) extracts against 1,3-DCP-treated cells were also investigated. First, the activities of superoxide dismutase (SOD) and catalase (CAT) were diminished by the treatment of 1,3-DCP. Moreover, 1,3-DCP stimulated the expression and catalytic activity of cytochrome P450 2E1 (CYP2E1), an enzyme that generates reactive oxygen species in the liver. In contrast, co-treatment of 1,3-DCP with the extracts significantly decreased ROS generation and inhibited CYP2E1 activity without affecting its expression. The co-administration of extracts also restored the activities of SOD and CAT reduced by 1,3-DCP and protected against 1,3-DCP-mediated cell death. In conclusion, these results suggest that 1,3-DCP induces oxidative stress through the elevated CYP2E1 level, which is inhibited by the extracts, protecting cells against the effects of 1,3-DCP.
在本研究中，已证明1,3 - 二氯 - 2 - 丙醇（1,3 - DCP）可诱导人肝细胞HuH7发生氧化应激和细胞死亡。还研究了（）提取物和Uyeki（Uyeki）提取物对1,3 - DCP处理细胞的保护作用。首先，1,3 - DCP处理会降低超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。此外，1,3 - DCP会刺激细胞色素P450 2E1（CYP2E1）的表达和催化活性，该酶在肝脏中产生活性氧。相反，1,3 - DCP与提取物共同处理可显著减少活性氧的产生并抑制CYP2E1活性，而不影响其表达。提取物的共同给药还恢复了被1,3 - DCP降低的SOD和CAT的活性，并保护细胞免受1,3 - DCP介导的细胞死亡。总之，这些结果表明，1,3 - DCP通过升高CYP2E1水平诱导氧化应激，而提取物可抑制该水平，从而保护细胞免受1,3 - DCP的影响。
