Hwang Ji Eun, Suh Dong Hwa, Kim Kee-Tae, Paik Hyun-Dong
1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Republic of Korea.
2Bio/Molecular Informatics Center, Konkuk University, Seoul, 05029 Republic of Korea.
Food Sci Biotechnol. 2018 Aug 30;28(1):215-224. doi: 10.1007/s10068-018-0450-x. eCollection 2019 Feb.
Hydroponic ginseng (HPG) and soil-cultured ginseng (SCG) were extracted in 70% methanol to quantify relative content of 8 ginsenosides and polyphenolic compounds, and flavonoids to compare their antioxidative effects. Level of nitric oxide and inflammatory targets produced in LPS-stimulated RAW 264.7 cells were measured. 2-year-old HPG shoots contained highest levels of ginsenoside Rb2, Rb3, Rd, Re, and F1. Total polyphenol content was highest in shoots of HPG, followed by roots of HPG and SCG. HPG shoots had high radical scavenging activity and an elevated ability to inhibit linoleic acid oxidation. 2-year-old HPG shoots reduced nitric oxide production in RAW 264.7 cells by 47%, whereas 6-year-old SCG roots reduced it by only 21%. HPG also significantly lowered mRNA expression of iNOS, TNF-α, IL-1β, and IL-6, as determined by RT-PCR, compared to SCGs. Therefore, HPG may have potential for utilization as an alternative to SCG, because of superior antioxidant and anti-inflammatory properties.
水培人参（HPG）和土培人参（SCG）用70%甲醇提取，以定量8种人参皂苷、多酚化合物和黄酮类化合物的相对含量，比较它们的抗氧化作用。测定脂多糖刺激的RAW 264.7细胞中产生的一氧化氮水平和炎症靶点。2年生水培人参地上部分人参皂苷Rb2、Rb3、Rd、Re和F1的含量最高。总多酚含量在水培人参地上部分最高，其次是水培人参根和土培人参根。水培人参地上部分具有较高的自由基清除活性和抑制亚油酸氧化的能力。2年生水培人参地上部分使RAW 264.7细胞中一氧化氮的产生减少47%，而6年生土培人参根仅使其减少21%。与土培人参相比，通过逆转录聚合酶链反应测定，水培人参还显著降低了诱导型一氧化氮合酶、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6的mRNA表达。因此，由于具有优异的抗氧化和抗炎特性，水培人参可能有潜力作为土培人参的替代品加以利用。
