State Key Laboratory of BioControl, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, South China Sea Resource Exploitation and Protection Collaborative Innovation Center, School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Appl Microbiol Biotechnol. 2019 Apr;103(7):3111-3122. doi: 10.1007/s00253-019-09671-9. Epub 2019 Feb 27.
The intestinal microbiota plays crucial roles in host health. The Pacific white shrimp is one of the most profitable aquaculture species in the world. Antibiotic supplement in feed is an optional practice to treat shrimp bacterial diseases. However, little is known about antibiotic effects on intestinal microbiota in pacific white shrimp. Here, shrimps were given feed supplemented with ciprofloxacin (Cip) (40 and 80 mg kg) and sulfonamide (Sul) (200 and 400 mg kg) to investigate the microbial community by targeting the V4 region of 16S rRNA genes. Within 4 days after feeding with normal feed and with antibiotics, antibiotic concentrations of Cip and Sul groups in the intestine dropped sharply. Significantly, increased abundance of antibiotic resistance genes (ARGs) of ciprofloxacin (qnrB, qnrD, and qnrS) and sulfonamide (sul1, sul2, and sul3) was observed in Cip and Sul groups (P < 0.05). A total of 3191 operational taxonomic units (OTUs) were obtained and 41 phyla were identified from 63 samples in shrimp intestine. The numbers of OTUs and Shannon index decreased rapidly at day 1 (the first day after feeding with antibiotics) and increased at day 3 (the third day after feeding with antibiotics). The relative abundance of dominant phyla and genera in Cip and Sul groups were significantly different from that in the control group (Ctrl). Furthermore, functional potentials that were related to amino acid metabolism, carbohydrate metabolism, and cellular processes and signaling varied significantly in Cip and Sul groups. These results point to an antibiotic-induced shift in shrimp intestinal microbiota, which highlights the importance of considering the microbiota in shrimp health management.
肠道微生物群在宿主健康中起着至关重要的作用。凡纳滨对虾是世界上最具经济效益的水产养殖物种之一。在饲料中添加抗生素是治疗虾类细菌性疾病的一种可选方法。然而，人们对抗生素对凡纳滨对虾肠道微生物群的影响知之甚少。在这里，通过靶向 16S rRNA 基因的 V4 区，用添加环丙沙星（Cip）（40 和 80mgkg）和磺胺类（Sul）（200 和 400mgkg）的饲料喂养对虾，以研究微生物群落。在正常饲料和抗生素喂养后的 4 天内，肠道中环丙沙星和磺胺类药物组的抗生素浓度急剧下降。值得注意的是，在 Cip 和 Sul 组中观察到了对环丙沙星（qnrB、qnrD 和 qnrS）和磺胺类（sul1、sul2 和 sul3）的抗生素抗性基因（ARGs）的丰度显著增加（P<0.05）。从 63 个虾肠样本中获得了 3191 个操作分类单元（OTUs），并鉴定出 41 个门。OTUs 的数量和 Shannon 指数在第 1 天（抗生素喂养后的第 1 天）迅速下降，在第 3 天（抗生素喂养后的第 3 天）增加。Cip 和 Sul 组中优势门和属的相对丰度与对照组（Ctrl）显著不同。此外，与氨基酸代谢、碳水化合物代谢、细胞过程和信号转导相关的功能潜力在 Cip 和 Sul 组中差异显著。这些结果表明，抗生素会引起虾肠道微生物群的变化，这凸显了在虾健康管理中考虑微生物群的重要性。
