Yang Zi-Wen, Salam Nimaichand, Hua Zheng-Shuang, Liu Bing-Bing, Han Ming-Xian, Fang Bao-Zhu, Wang Dong, Xiao Min, Hozzein Wael N, Li Wen-Jun
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4862-4867. doi: 10.1099/ijsem.0.002397. Epub 2017 Oct 6.
Strain SYSU D8009 was isolated from a desert sample collected from Saudi Arabia. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, non-motile, aerobic and non-spore-forming. It was able to grow at 4-45 °C and pH 4.0-8.0, and exhibited NaCl tolerance of up to 1.5 % (w/v). Strain SYSU D8009 shared the closest 16S rRNA gene sequence similarities with members of the family Acetobacteraceae, with a value of less than 96.0 %. In the phylogenetic dendrograms, the strain clustered with the genera Paracraurococcus, Craurococcus and Crenalkalicoccus within the family Acetobacteraceae but with a distinct lineage, thereby demonstrating that the strain should be classified within the family Acetobacteraceae. The respiratory ubiquinone was found to be Q-10. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminolipids. The predominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The genomic DNA G+C content of strain SYSU D8009 was determined to be 71.6 mol%. Based on the results of the phylogenetic analyses and differences in the physiological and biochemical characteristics, strain SYSU D8009 merits representation of a novel species of a new genus within the family Acetobacteraceae, for which the name Siccirubricoccus deserti gen. nov., sp. nov. is proposed. The type strain of Siccirubricoccus deserti sp. nov. is SYSU D8009 (=CGMCC 1.15936=KCTC 62088).
菌株SYSU D8009是从沙特阿拉伯采集的沙漠样本中分离得到的。采用多相分类法对该分离株的分类地位进行了研究。该新型分离株革兰氏染色阴性，无运动性，好氧，不产芽孢。它能够在4-45°C和pH 4.0-8.0条件下生长，对NaCl的耐受性高达1.5%（w/v）。菌株SYSU D8009与醋杆菌科成员的16S rRNA基因序列相似度最高，但低于96.0%。在系统发育树状图中，该菌株与醋杆菌科内的副红球菌属、红球菌属和嗜碱球菌属聚类，但形成一个独特的分支，从而表明该菌株应归类于醋杆菌科。发现呼吸醌为Q-10。该菌株的极性脂质包括双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和四种未鉴定的氨基脂质。主要的细胞脂肪酸为总和特征8（C18:1ω7c和/或C18:1ω6c）和C16:0。菌株SYSU D8009的基因组DNA G+C含量测定为71.6 mol%。基于系统发育分析结果以及生理生化特征的差异，菌株SYSU D8009应代表醋杆菌科内一个新属的新物种，为此提出新属名为沙漠红球菌属（Siccirubricoccus），新物种名为沙漠红球菌（Siccirubricoccus deserti）。沙漠红球菌新物种的模式菌株为SYSU D8009（=CGMCC 1.15936=KCTC 62088）。
