Kawakami Daichi, Yoshida Takanobu, Kanemaru Yutaro, Huarhua Zaquinaula Medali Heidi, Mizukami Tomomichi, Arimoto Michiko, Shibata Takahiro, Goto Akihiro, Enami Yoshinari, Amano Hiroshi, Teraoka Tohru, Komatsu Ken, Arie Tsutomu
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
J Pestic Sci. 2019 Feb 20;44(1):41-47. doi: 10.1584/jpestics.D18-064.
Ultrasound, which refers to frequencies above the audible limit of human hearing, is a candidate for inducing resistance to pathogens in plants. We revealed that aerial ultrasound of 40.5 kHz could induce disease resistance in tomatoes and rice when the plants were irradiated with ultrasound of 100 dB for 2 weeks during nursery season and reduced the incidence of Fusarium wilt and blast diseases, respectively, when plants were inoculated with pathogen 0 or 1 week after terminating irradiation. Disease control efficacy was also observed with ultrasound at frequencies of 19.8 and 28.9 kHz. However, cabbage yellows and powdery mildew on lettuce were not suppressed by ultrasound irradiation. No significant positive or negative effect on growth was observed in tomato and rice plants. RT-qPCR showed that the expression of involved in the salicylic acid (SA) signaling pathway was upregulated in the ultrasound-irradiated tomato.
超声波是指频率高于人类听力可听极限的声波，它是诱导植物对病原体产生抗性的一个候选因素。我们发现，在育苗期对番茄和水稻植株进行2周的100分贝40.5千赫的空气超声波照射，可诱导其产生抗病性，在停止照射后0或1周接种病原体时，分别降低了枯萎病和稻瘟病的发病率。在19.8和28.9千赫频率的超声波照射下也观察到了病害防治效果。然而，超声波照射并未抑制甘蓝黄化病和生菜白粉病。在番茄和水稻植株中未观察到对生长有显著的正面或负面影响。逆转录定量聚合酶链反应（RT-qPCR）表明，在超声波照射的番茄中，参与水杨酸（SA）信号通路的基因表达上调。
