Arts and Science Faculty, Department of Chemistry, Suleyman Demirel University, Cunur, 32260, Isparta, Turkey.
Institute of Science, Department of Molecular Biology and Genetics, Istanbul University, Fatih, 34134, Istanbul, Turkey.
Appl Biochem Biotechnol. 2019 Aug;188(4):1145-1157. doi: 10.1007/s12010-019-02979-2. Epub 2019 Feb 28.
Polypyrrole (PPy) and PPy-containing chitosan-coated FeO have been electrochemically polymerized on pencil graphite electrodes (PGEs). After the resulting electrodes were characterized by SEM-EDS analysis, glucose-6-phosphate dehydrogenase (G6PD) was immobilized onto these electrodes via glutaraldehyde. The biosensors prepared for the chronopotentiometric detection of glucose-6-phosphate (G6P) at 0.25 mAcm were studied and optimized at different parameters such as the pH of supporting electrolyte, the temperature, and NADP and G6P concentrations related with the analytical performance of the biosensors. PPy/G6PD (BS-1) and CS/FeO-PPy/G6PD (BS-2) biosensors showed a broad linear response in the concentration range 0.025-0.25 mM and 0.0025-0.05 mM, and their detection limits for G6P and the RSD values were determined as 0.008 mM and 0.002 mM and 3.80% and 4.60% after 15 times usage, respectively. The interference study with various major blood components such as urea, glucose, and cysteine was performed to evaluate the selectivity of the biosensors. The proposed BS-2 biosensor showed almost free response from available interferences in blood serum with a recovery of 91 to 110%. The developed biosensors could be used in the G6P level measurement of medical samples.
聚吡咯（PPy）和壳聚糖包覆的 FeO 中含有 PPy 已在铅笔石墨电极（PGE）上通过电化学聚合。对所得电极进行 SEM-EDS 分析后，通过戊二醛将葡萄糖-6-磷酸脱氢酶（G6PD）固定在这些电极上。通过 chronopotentiometric 法研究了用于检测葡萄糖-6-磷酸（G6P）的生物传感器，在 0.25 mAcm 下优化了不同参数，如支持电解质的 pH 值、温度以及与生物传感器分析性能相关的 NADP 和 G6P 浓度。PPy/G6PD（BS-1）和 CS/FeO-PPy/G6PD（BS-2）生物传感器在 0.025-0.25 mM 和 0.0025-0.05 mM 的浓度范围内表现出宽线性响应，其对 G6P 的检测限和 RSD 值分别为 0.008 mM 和 0.002 mM 和 3.80%和 4.60%，在 15 次使用后。进行了各种主要血液成分（如尿素、葡萄糖和半胱氨酸）的干扰研究，以评估生物传感器的选择性。所提出的 BS-2 生物传感器在血清中具有几乎不受可用干扰影响的响应，回收率为 91%至 110%。开发的生物传感器可用于医学样本中 G6P 水平的测量。
