Margolis J, Eisen M
Vox Sang. 1986;50(1):38-41. doi: 10.1111/j.1423-0410.1986.tb04843.x.
A procedure for preparing single units of freeze-dried cryoprecipitate (cryo) in the original bags is described in detail. Batches of frozen plasma bags were rapidly thawed by rocking in a water bath, centrifuged and after complete removal of supernate the compact cryo pellets (2-5 g) were lyophilized with the aid of a holding tray with a filtered air chamber designed for this purpose. Adding synthetic amino acids has greatly improved stability of the dried concentrate during storage and has increased its resistance to heat denaturation (e.g. less than 10% loss of potency after 20 h at 70 degrees C). The method is presented as a viable option for safe and economical manufacture of a stable factor VIII concentrate.
详细描述了一种在原始袋子中制备冻干冷沉淀（cryo）单单位的方法。将一批冷冻血浆袋在水浴中摇晃快速解冻，离心，完全去除上清液后，借助为此设计的带有过滤空气腔的保持盘对致密的冷沉淀颗粒（2 - 5克）进行冻干。添加合成氨基酸极大地提高了干燥浓缩物在储存期间的稳定性，并增强了其耐热变性能力（例如，在70摄氏度下20小时后效力损失小于10%）。该方法被认为是安全、经济地生产稳定的凝血因子VIII浓缩物的可行选择。
