Arzmi Mohd Hafiz, Alnuaimi Ali D, Dashper Stuart, Cirillo Nicola, Reynolds Eric C, McCullough Michael
Oral Health CRC, Melbourne Dental School, The University of Melbourne, Victoria, Australia Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan, Pahang, Malaysia.
Oral Health CRC, Melbourne Dental School, The University of Melbourne, Victoria, Australia.
Med Mycol. 2016 Nov 1;54(8):856-64. doi: 10.1093/mmy/myw042. Epub 2016 Jun 26.
Oral biofilms comprise of extracellular polysaccharides and polymicrobial microorganisms. The objective of this study was to determine the effect of polymicrobial interactions of Candida albicans, Actinomyces naeslundii, and Streptococcus mutans on biofilm formation with the hypotheses that biofilm biomass and metabolic activity are both C. albicans strain and growth medium dependent. To study monospecific biofilms, C. albicans, A. naeslundii, and S. mutans were inoculated into artificial saliva medium (ASM) and RPMI-1640 in separate vials, whereas to study polymicrobial biofilm formation, the inoculum containing microorganisms was prepared in the same vial prior inoculation into a 96-well plate followed by 72 hours incubation. Finally, biofilm biomass and metabolic activity were measured using crystal violet and XTT assays, respectively. Our results showed variability of monospecies and polymicrobial biofilm biomass between C. albicans strains and growth medium. Based on cut-offs, out of 32, seven RPMI-grown biofilms had high biofilm biomass (HBB), whereas, in ASM-grown biofilms, 14 out of 32 were HBB. Of the 32 biofilms grown in RPMI-1640, 21 were high metabolic activity (HMA), whereas in ASM, there was no biofilm had HMA. Significant differences were observed between ASM and RPMI-grown biofilms with respect to metabolic activity (P <01). In conclusion, biofilm biomass and metabolic activity were both C. albicans strain and growth medium dependent.
口腔生物膜由细胞外多糖和多种微生物组成。本研究的目的是确定白色念珠菌、内氏放线菌和变形链球菌的多微生物相互作用对生物膜形成的影响，假设生物膜生物量和代谢活性均依赖于白色念珠菌菌株和生长培养基。为了研究单菌种生物膜，将白色念珠菌、内氏放线菌和变形链球菌分别接种到单独小瓶中的人工唾液培养基（ASM）和RPMI-1640中，而要研究多菌种生物膜形成，则在接种到96孔板之前，先在同一小瓶中制备含有微生物的接种物，然后孵育72小时。最后，分别使用结晶紫和XTT试验测量生物膜生物量和代谢活性。我们的结果表明，白色念珠菌菌株和生长培养基之间的单菌种和多菌种生物膜生物量存在差异。根据临界值，在32个样本中，7个在RPMI中培养的生物膜具有高生物膜生物量（HBB），而在ASM中培养的生物膜中，32个中有14个是HBB。在RPMI-1640中培养的32个生物膜中，21个具有高代谢活性（HMA），而在ASM中，没有生物膜具有HMA。在代谢活性方面，ASM和RPMI中培养的生物膜之间观察到显著差异（P<0.01）。总之，生物膜生物量和代谢活性均依赖于白色念珠菌菌株和生长培养基。
