Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China.
Int J Mol Sci. 2019 Mar 1;20(5):1064. doi: 10.3390/ijms20051064.
Uridine diphosphate glycosyltransferases (UGTs) are multifunctional detoxification enzymes, which are involved in metabolizing various chemicals and contribute to the development of insecticide resistance. However, the possible roles of UGTs in chlorantraniliprole resistance in have rarely been studied in detail. Based on genome data, 24 UGT genes in belonging to 11 families were identified, which were designated by the UGT nomenclature committee. Synergism assay data suggested that UGTs are potentially involved in chlorantraniliprole resistance in and were significantly overexpressed in the chlorantraniliprole resistant strain (12.36- and 5.34-fold, respectively). The two UGTs were highly expressed in the larval Malpighian tubules, fat body, and midgut; however, expression was lowest in the head. Injection of individual dsRNAs reduced the expression of the two target genes (by 69.34% and 48.74%, respectively) and caused significant higher larval mortality (81.33% and 54.67%, respectively). Overexpression of and was potentially involved in chlorantraniliprole resistance in as confirmed by the RNAi assay. Our findings suggest that overexpression of UGTs may contribute to chlorantraniliprole resistance in .
尿苷二磷酸糖基转移酶（UGTs）是多功能解毒酶，参与代谢各种化学物质，并有助于杀虫剂抗性的发展。然而，UGTs 在 中氯虫苯甲酰胺抗性中的可能作用在很大程度上尚未得到详细研究。基于基因组数据，在 中鉴定出属于 11 个家族的 24 个 UGT 基因，这些基因由 UGT 命名委员会指定。增效作用测定数据表明，UGTs 可能参与 中氯虫苯甲酰胺抗性，并且在氯虫苯甲酰胺抗性菌株中显著过表达（分别为 12.36 倍和 5.34 倍）。这两个 UGT 在幼虫的马尔皮基氏小管、脂肪体和中肠中高度表达；然而，在头部表达最低。单独注射 dsRNA 可降低两个靶基因的表达（分别降低 69.34%和 48.74%），并导致幼虫死亡率显著升高（分别为 81.33%和 54.67%）。RNAi 测定证实， 和 的过表达可能参与了 中氯虫苯甲酰胺的抗性。我们的研究结果表明，UGTs 的过表达可能有助于 对氯虫苯甲酰胺的抗性。
