Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, Saltillo, Coahuila 25280, Mexico.
Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, Saltillo, Coahuila 25280, Mexico; Cluster of Bioalcohols, Mexican Centre for Innovation in Bioenergy (Cemie-Bio), Mexico.
Carbohydr Polym. 2019 May 1;211:349-359. doi: 10.1016/j.carbpol.2019.01.111. Epub 2019 Feb 8.
One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 °C/30 min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.
从木质纤维素生物质生产生物燃料的主要挑战之一是在预处理生物质的酶解阶段从纤维素中产生高葡萄糖浓度，以保证具有成本效益的工艺。因此，在高固体负荷下对纤维素进行酶糖化是一种替代方法。在这项工作中，龙舌兰蔗渣在 194°C/30 分钟的条件下进行水热预处理和优化，得到富含纤维素（>46.46%）的预处理固体，并在高固体水平下进行酶水解。设计了一个水平式生物反应器用于高固体负荷下的酶糖化[25%（w/v）]。该生物反应器提高了混合效率，纤维素转化率高达 98%（72 小时时为 195.6 g/L）。此外，纤维素酶失活动力学的数学建模表明，纤维素酶在反应的最初几个小时内失去了大部分初始活性。还通过 X 射线衍射对纤维素进行了表征，并使用扫描电子显微镜观察了预处理后的固体。
