Biorefinery Group, Food Research Department, Faculty of Chemistry Sciences, Autonomous University of Coahuila, Saltillo, Coahuila 25280, Mexico; Cluster of Bioalcohols, Mexican Centre for Innovation in Bioenergy (Cemie-Bio), Mexico.
Universidad Autónoma Agraria Antonio Narro, 1923 Antonio Narro St., Buenavista, Saltillo, Coahuila 25315, Mexico.
Bioresour Technol. 2018 Sep;263:112-119. doi: 10.1016/j.biortech.2018.04.100. Epub 2018 Apr 30.
The production of tequila in Mexico generates a large amount of agave bagasse per year. However, this biomass can be considered as a potential source for biofuel production. In this study, it is described how the hydrothermal pretreatment was scaled in a bench scale, considering the severity index as a strategy. The best condition was at 180 °C in isothermal regime for 20 min with 65.87% of cellulose content and high concentration of xylooligosaccharides (15.31 g/L). This condition was scaled up (using severity factor: [logR] = 4.11), in order to obtain a rich pretreated solid in cellulose to perform the enzymatic hydrolysis, obtaining saccharification yields of 98.5 and 99.5% at high-solids loading (10 and 15%, respectively). The pre-saccharification and fermentation strategy was used in the bioethanol production at 10 and 15% of total pretreated solids, obtaining 38.39 and 55.02 g/L of ethanol concentration, corresponding to 90.84% and 87.56% of ethanol yield, respectively.
在墨西哥，龙舌兰酒的生产每年都会产生大量的龙舌兰纤维废渣。然而，这种生物质可以被视为生物燃料生产的潜在来源。本研究描述了如何通过热预处理在台架规模上进行放大，将苛刻指数作为一种策略。最佳条件是在 180°C 下恒温处理 20 分钟，纤维素含量为 65.87%，木二糖含量较高（15.31g/L）。在此条件下进行放大（使用苛刻因子：[logR] = 4.11），以获得富含纤维素的预处理固体，用于进行酶水解，在高固体负载（分别为 10%和 15%）下可获得 98.5%和 99.5%的糖化收率。在总预处理固体的 10%和 15%下采用预糖化和发酵策略进行生物乙醇生产，可获得 38.39 和 55.02g/L 的乙醇浓度，分别对应于 90.84%和 87.56%的乙醇得率。
