Hu Chengjun, Xing Weigang, Liu Xiaohua, Zhang Xiuzhu, Li Ke, Liu Jia, Deng Baichuan, Deng Jinping, Li Yong, Tan Chengquan
Guangdong Provincial Key Laboratory of Animal Nutrition Control, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou, 510640, China.
Newhope Liuhe Corp. Ltd, Beijing, 100102, People's Republic of China.
AMB Express. 2019 Mar 1;9(1):33. doi: 10.1186/s13568-019-0755-z.
The adverse effects of antibiotics have attracted widespread attention, thus reducing the use of antibiotics in animal feed has become a very important issue in improving of the health of livestock. The effects of Enterococcus faecium (E. faecium) on growth performance and gut microbiota in weaned piglets were investigated in the present study. Piglets were randomly assigned to four treatments: a control group fed with a diet containing 75 mg/kg aureomycin (Diet 1 group) and three experimental groups fed with diets of 50 mg/kg aureomycin (Diet 2 group), 50 mg/kg aureomycin + 9 × 10 CFU/g E. faecium (Diet 3 group), or 50 mg/kg aureomycin + 1.2 × 10 CFU/g E. faecium (Diet 4 group). Their gut microbial communities were analyzed by sequencing the V3-V4 region of the 16SrRNA gene. The results showed that the final body weights and the average daily gain of the weaned piglets in the Diet 2 group were higher (P = 0.05) than those in the Diet 1 or Diet 3 group. Decreasing trends (P = 0.08) was observed in mortality rate in the Diet 3 and 4 group when compared with that in the Diet 1 group. Increases in the Sobs, Chao1, ACE, and Shannon indexes and a decrease in the Simpson index were observed at intervals from day 1 to 14 (P < 0.05). The Sobs, Chao1, and ACE indexes in the Diet 3 group were the lowest on day 14 (P < 0.05). The abundance of Bacteroidetes was increased and that of Proteobacteria was decreased from day 1 to 7, but both of them kept stable from day 7 to 14. Besides, the lowest abundance of Fusobacteria, Lentisphaerae, and Planctomycetes was observed on day 1 and the lowest abundance of Actinobacteria was observed on day 14 in the Diet 3 group (P < 0.05). Overall, these results suggest that the antibiotics and E. faecium interventions result in different changes in the gut microbiota, and a reduced antibiotics diet supplemented with 1.2 × 10 CFU/g E. faecium does not affect the growth performance in weaned piglets.
抗生素的副作用已引起广泛关注，因此减少抗生素在动物饲料中的使用已成为改善家畜健康的一个非常重要的问题。本研究调查了屎肠球菌（E. faecium）对断奶仔猪生长性能和肠道微生物群的影响。仔猪被随机分为四组处理：对照组饲喂含75 mg/kg金霉素的日粮（日粮1组），三个实验组分别饲喂含50 mg/kg金霉素的日粮（日粮2组）、50 mg/kg金霉素 + 9×10⁸ CFU/g屎肠球菌的日粮（日粮3组）或50 mg/kg金霉素 + 1.2×10⁹ CFU/g屎肠球菌的日粮（日粮4组）。通过对16SrRNA基因的V3 - V4区域进行测序来分析它们的肠道微生物群落。结果表明，日粮2组断奶仔猪的末体重和平均日增重高于（P = 0.05）日粮1组或日粮3组。与日粮1组相比，日粮3组和日粮4组的死亡率呈下降趋势（P = 0.08）。从第1天到第14天期间，观察到Sobs、Chao1、ACE和Shannon指数增加，Simpson指数下降（P < 0.05）。日粮3组在第14天的Sobs、Chao1和ACE指数最低（P < 0.05）。从第1天到第7天，拟杆菌门的丰度增加，变形菌门的丰度降低，但从第7天到第14天它们都保持稳定。此外，日粮3组在第1天观察到梭杆菌门、浮霉菌门和扁平菌门的丰度最低，在第14天观察到放线菌门的丰度最低（P < 0.05）。总体而言，这些结果表明抗生素和屎肠球菌干预导致肠道微生物群发生不同变化，并且补充1.2×10⁹ CFU/g屎肠球菌的低抗生素日粮不会影响断奶仔猪的生长性能。
