1 College of Animal Science, Zhejiang University, Hangzhou 310058, China P.R.
2 College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China P.R.
Benef Microbes. 2016 Sep;7(4):529-38. doi: 10.3920/BM2015.0099. Epub 2016 Apr 19.
The oral administration of Enterococcus faecium EF1 to new-born suckling and weaning piglets along with their growth performances and intestinal microbiota was investigated in this study. Twenty-four new-born piglets were initially divided into 2 groups. The probiotics group received 2 ml of 10% sterilised skimmed milk by oral gavage supplemented with 6×10(8) cfu/ml viable E. faecium EF1 at the first, the third and the fifth day after birth, while the control group received 2 ml of 10% sterilised skimmed milk without probiotics at the same time. Results showed that oral administration of E. faecium EF1 was associated with a remarkable increase on the body weight of piglets for both suckling and weaning periods, by 30.73% (P<0.01) and 320.84% (P<0.01), and also decreased the diarrhoea rate, by 43.21% (P<0.05) and 71.42% (P<0.05), respectively. In addition, 454-pyrosequencing analysis revealed that there was no significant difference in the intestinal microbial diversity of the suckling piglets between the two groups; nevertheless, when compared to the control group, the relative abundance of Firmicutes in the probiotics group was substantially augmented, while the relative abundance of Proteobacteria, Bacteroidetes and Fusobacteria diminished. However, results indicated that oral administration of E. faecium EF1 did not have any influence on the relative abundance of Firmicutes in weaning piglets rather than increasing the relative abundance of Bacteroidetes and decreasing the relative abundance of Proteobacteria. Furthermore, at the level of the Firmicutes phylum, the relative abundance of Lactobacillales in the probiotic group increased significantly. These findings suggest that oral administration of E. faecium EF1 to new-born piglets could improve the growth performance and intestinal microbiota of piglets for both suckling and weaning periods.
本研究旨在探讨屎肠球菌 EF1 对新生哺乳仔猪和断奶仔猪的口服给药及其生长性能和肠道微生物群的影响。24 头新生仔猪最初分为 2 组。益生菌组在出生后第 1、3 和 5 天分别通过口服灌胃接受 2 ml 的 10%无菌脱脂奶，其中含有 6×10(8)cfu/ml 活屎肠球菌 EF1，而对照组同时接受 2 ml 的 10%无菌脱脂奶而不添加益生菌。结果表明，口服屎肠球菌 EF1 可使哺乳和断奶期仔猪的体重显著增加，分别增加 30.73%（P<0.01）和 320.84%（P<0.01），同时降低腹泻率，分别降低 43.21%（P<0.05）和 71.42%（P<0.05）。此外，454 焦磷酸测序分析显示，两组哺乳仔猪的肠道微生物多样性无显著差异；然而，与对照组相比，益生菌组的厚壁菌门相对丰度显著增加，而变形菌门、拟杆菌门和梭杆菌门的相对丰度则降低。然而，结果表明，口服屎肠球菌 EF1 对断奶仔猪厚壁菌门的相对丰度没有影响，反而增加了拟杆菌门的相对丰度，降低了变形菌门的相对丰度。此外，在厚壁菌门水平上，益生菌组的乳杆菌科相对丰度显著增加。这些发现表明，口服屎肠球菌 EF1 可改善新生仔猪的生长性能和肠道微生物群，无论是在哺乳还是断奶期。
