Guevarra Robin B, Kim Eun Sol, Cho Jin Ho, Song Minho, Cho Jae Hyoung, Lee Jun Hyung, Kim Hyeri, Kim Sheena, Keum Gi Beom, Lee Chan Ho, Cho Won Tak, Watthanaphansak Suphot, Kim Hyeun Bum
Department of Animal Resources Science, Dankook University, Cheonan, Republic of Korea.
Division of Food and Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
Front Vet Sci. 2023 Jan 12;9:1101869. doi: 10.3389/fvets.2022.1101869. eCollection 2022.
Development of alternatives to in-feed antibiotics in the swine industry have been the focused of many pig gut microbiota studies to improve animal health. In this study, we evaluated the effects of probiotic (PRO), prebiotic lactulose (PRE), and their synbiotic combination (SYN) on gut microbiota using 16S rRNA gene sequencing in weaned piglets challenged with Shiga-toxin producing (STEC). Our data showed that prebiotics, probiotics and synbiotics improved the intestinal health in weaned piglets. No significant differences were observed in species richness and species diversity in weaned piglets fed prebiotics, probiotics and their synbiotic combination. However, beta diversity analysis revealed distinct clustering of the microbiota of according to dietary treatment and by oral challenge of STEC. At the phylum level, Firmicutes to Bacteroidetes ratio was lower in the dietary treatment groups than the control group. Oral supplementation of prebiotics, probiotics and synbiotics enriched the abundance of and . was elevated in PRO group; however, was depleted with STEC challenge regardless of dietary treatment. Overall, our data showed that administration of synbiotics in piglets improved intestinal health through gut microbiota modulation. Our data indicated that prebiotics, probiotics and their synbiotic combination could promote intestinal health through gut microbiota modulation in weaned piglets.
猪产业中饲料抗生素替代品的开发一直是许多猪肠道微生物群研究的重点，目的是改善动物健康。在本研究中，我们使用16S rRNA基因测序评估了益生菌（PRO）、益生元乳果糖（PRE）及其合生元组合（SYN）对感染产志贺毒素大肠杆菌（STEC）的断奶仔猪肠道微生物群的影响。我们的数据表明，益生元、益生菌和合生元可改善断奶仔猪的肠道健康。在喂食益生元、益生菌及其合生元组合的断奶仔猪中，未观察到物种丰富度和物种多样性的显著差异。然而，β多样性分析显示，根据饮食处理和STEC口服挑战，微生物群存在明显的聚类。在门水平上，饮食处理组的厚壁菌门与拟杆菌门的比例低于对照组。口服补充益生元、益生菌和合生元可增加 和 的丰度。PRO组中 升高；然而，无论饮食处理如何，STEC挑战都会使其减少。总体而言，我们的数据表明，在仔猪中施用合生元可通过调节肠道微生物群来改善肠道健康。我们的数据表明，益生元、益生菌及其合生元组合可通过调节断奶仔猪的肠道微生物群来促进肠道健康。
