基于荧光的小分子生物传感器的设计和优化的结构和进化方法。
Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors.
机构信息
Research School of Chemistry, The Australian National University, 137 Sullivans Creek Road, Acton, ACT 2601, Australia.
Research School of Chemistry, The Australian National University, 137 Sullivans Creek Road, Acton, ACT 2601, Australia.
出版信息
Curr Opin Struct Biol. 2019 Aug;57:31-38. doi: 10.1016/j.sbi.2019.01.013. Epub 2019 Feb 27.
Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.
生物传感器可以选择性地报告特定小分子分析物的存在，在许多研究、医学和生物技术领域都有应用。在这里，我们回顾了基于遗传编码荧光的小分子生物传感器的设计和优化方面的最新进展和新兴方法。我们讨论了如何利用天然感应蛋白来产生新型生物传感器，以及优化配体特异性和荧光读数的策略。最后，我们深入探讨了高通量传感器优化，并讨论了在设计新型生物传感器时所面临的挑战。
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