Sinel'shchikova T A, L'vova G N, Shoniia N N, Zasukhina G D
Genetika. 1986 Feb;22(2):322-7.
DNA repair activity in cells after gamma-irradiation or treatment with 4-nitroguinoline-1-oxide was studied by alkaline elution of cells lysed on membrane filters, as well as by reactivation and induced mutagenesis of vaccinia virus in lymphocytes and fibroblasts obtained in skin biopsy from patients with homocystinuria. Disorders of the repair were demonstrated in lymphocytes by using both criteria. In all fibroblast passages repair disorder was only found for gamma-irradiation, the repair activity being comparable with that of lymphocytes. The level of spontaneous and gamma-induced mutations in vaccinia virus propagated in fibroblasts was increased at later culture passages.
通过对膜滤器上裂解细胞进行碱性洗脱，以及通过对同型胱氨酸尿症患者皮肤活检获得的淋巴细胞和成纤维细胞中痘苗病毒的再激活和诱导诱变，研究了γ射线照射或用4-硝基喹啉-1-氧化物处理后细胞中的DNA修复活性。使用这两种标准均证明淋巴细胞存在修复障碍。在所有成纤维细胞传代中，仅发现γ射线照射存在修复障碍，其修复活性与淋巴细胞相当。在后期培养传代中，在成纤维细胞中繁殖的痘苗病毒的自发突变和γ诱导突变水平增加。
