L'vova G N, Sinel'shchikova T A, Zasukhina G D
Genetika. 1996 Jun;32(6):860-2.
Two lines of fibroblasts isolated from patients with homocystinuria were characterized by the test of vaccinia virus host-cell reactivation. Xeroderma pigmentosum cells and normal fibroblasts were used as a control. Reduced host cell reactivation of the virus and an enhanced level of induced virus mutations in comparison with normal cells were revealed in homocystinuria cells after 4NQO and gamma-ray treatment. Enhanced reactivation and a corresponding reduced level of gamma-induced mutagenesis were found in preirradiated normal cells and in the xeroderma pigmentosum cells. The level of enhanced reactivation of the virus was virtually the same in preirradiated and nonirradiated homocystinuria cells, while the level of induced mutagenesis was reduced in both cells. This indicates the difference in the capability of the virus system for enhanced reactivation to repair potentially lethal and premutational DNA damages.
通过痘苗病毒宿主细胞复活试验对从同型胱氨酸尿症患者分离出的两株成纤维细胞进行了特性分析。以着色性干皮病细胞和正常成纤维细胞作为对照。在经4 - 硝基喹啉氧化物（4NQO）和γ射线处理后，与正常细胞相比，同型胱氨酸尿症细胞中病毒的宿主细胞复活能力降低，且诱导的病毒突变水平升高。在预先照射的正常细胞和着色性干皮病细胞中发现复活能力增强，γ诱导的诱变水平相应降低。预先照射和未照射的同型胱氨酸尿症细胞中病毒复活增强的水平基本相同，而两种细胞中诱导的诱变水平均降低。这表明病毒系统在增强复活以修复潜在致死性和前突变性DNA损伤的能力方面存在差异。
