Beilby M J
School of Physics, University of NSW, Kensington, NSW, 2052, Australia.
Biophys Rev. 2019 Apr;11(2):235-239. doi: 10.1007/s12551-019-00512-7. Epub 2019 Mar 4.
The large-celled green alga Chara provided early electrophysiological data, but this model organism lost popularity once the smaller cells of higher plants became accessible to electrophysiology and genetic manipulation. However, with the sequencing of the Chara braunii genome (Nishiyama et al. Cell 174: 448-464, 2018), the molecular identity of the underlaying ion transporters in Characeae can be found and placed in evolutionary context. As Characeae are close to ancestors of land plants, the wealth of electrophysiological data will provide insights into important aspects of plant physiology, such as salt tolerance and sensitivity, carbon concentrating mechanisms, pH banding and the action potential generation.
大型绿藻轮藻提供了早期的电生理数据,但一旦高等植物的较小细胞能够用于电生理学和基因操作,这种模式生物就不再受欢迎。然而,随着布氏轮藻基因组的测序(西山等人,《细胞》174: 448 - 464, 2018),可以找到轮藻科中潜在离子转运蛋白的分子身份,并将其置于进化背景中。由于轮藻科接近陆地植物的祖先,丰富的电生理数据将为植物生理学的重要方面提供见解,如耐盐性和敏感性、碳浓缩机制、pH带以及动作电位的产生。