Makkouk K M, Ghulam W, Kumari S G
Virology Laboratory, Germplasm Program, International Center for Agricultural Research in the Dry Areas (ICARDA), P. O. Box 5466, Aleppo, Syria.
Plant Dis. 2001 Apr;85(4):446. doi: 10.1094/PDIS.2001.85.4.446A.
Symptoms suggestive of virus infection in barley, bread wheat, and durum wheat were observed at high incidence in November 2000 in Terbol, Beqa'a Valley, Lebanon. The symptoms were mainly stunting, accompanied by leaf striping and yellowing. Symptomatic plant samples (27 barley, 37 bread wheat, and 81 durum wheat) were collected and tested for the presence of four different viruses by tissue-blot immunoassay (TBIA) (1) at the Virology Laboratory of ICARDA, Aleppo, Syria. Antisera used were for Barley stripe mosaic virus (BSMV, genus Hordeivirus) (2); Barley yellow dwarf virus (BYDV, genus Luteovirus, family Luteoviridae) (PAV serotype) (2); Wheat streak mosaic virus (WSMV, genus Tritimovirus, family Potyviridae) (3); and Barley yellow striate mosaic virus (BYSMV, genus Cytorhabdovirus, family Rhabdoviridae) provided by M. Conti, Instituto di Fitovirologia applicata, Turino, Italy. BYSMV was detected in 12 barley, 18 bread wheat, and 56 durum wheat samples; the corresponding numbers of barley, bread wheat, and durum wheat plants testing positive for BYDV-PAV were 4, 7, and 6, respectively. BSMV and WSMV were not detected in any of the samples tested. BYSMV was purified from infected wheat plants, and the purified preparation had a UV 260:280 ratio of 1.18, typical of Rhabdoviruses. In SDS-polyacrylamide gel electrophoresis, the purified virus preparation indicated the presence of 66, 47, and 15 kDa structural proteins, typical of the G, N and M proteins of Rhabdoviruses. In western blot, the 66 and 47 kDa protein bands reacted strongly with BYSMV antiserum. This is the first record of BYSMV infecting barley and wheat in Lebanon. References: (1) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994. (2) K. M. Makkouk and S. G. Kumari. Rachis Newsl. 12:24, 1993. (3) K. M. Makkouk and S. G. Kumari. Rachis Newsl. 16:74, 1997.
2000年11月，在黎巴嫩贝卡谷地的特尔博尔，大麦、面包小麦和硬粒小麦上出现了高发病率的疑似病毒感染症状。症状主要为植株矮化，并伴有叶片条纹和黄化。采集了有症状的植株样本（27株大麦、37株面包小麦和81株硬粒小麦），并在叙利亚阿勒颇的国际干旱地区农业研究中心病毒学实验室通过组织印迹免疫分析（TBIA）(1)检测了四种不同病毒的存在情况。所用抗血清针对大麦条纹花叶病毒（BSMV，大麦病毒属）(2)；大麦黄矮病毒（BYDV，黄症病毒属，黄症病毒科）（PAV血清型）(2)；小麦线条花叶病毒（WSMV，小麦病毒属，马铃薯Y病毒科）(3)；以及由意大利都灵应用植物病毒学研究所的M. 孔蒂提供的大麦黄条花叶病毒（BYSMV，细胞质弹状病毒属，弹状病毒科）。在12株大麦、18株面包小麦和56株硬粒小麦样本中检测到了BYSMV；检测出BYDV - PAV呈阳性的大麦、面包小麦和硬粒小麦植株数量分别为4株、7株和6株。在所检测的任何样本中均未检测到BSMV和WSMV。从受感染的小麦植株中纯化出了BYSMV，纯化制剂的紫外260:280比值为1.18，这是弹状病毒的典型特征。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中，纯化的病毒制剂显示存在66 kDa、47 kDa和15 kDa的结构蛋白，这是弹状病毒G、N和M蛋白的典型特征。在蛋白质免疫印迹中，66 kDa和47 kDa的蛋白条带与BYSMV抗血清发生强烈反应。这是BYSMV在黎巴嫩感染大麦和小麦的首次记录。参考文献：(1) K. M. 马库克和A. 科莫。《欧洲植物病理学杂志》100:71，1994年。(2) K. M. 马库克和S. G.库玛里。《穗轴通讯》12:24，1993年。(3) K. M. 马库克和S. G.库玛里。《穗轴通讯》16:74，1997年。
