Vanden-Hehir Sally, Tipping William J, Lee Martin, Brunton Valerie G, Williams Anna, Hulme Alison N
EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh EH9 3FJ, UK.
Edinburgh Cancer Research UK Centre, University of Edinburgh, Western General Hospital, Crewe Road South, Edinburgh EH4 2XR, UK.
Nanomaterials (Basel). 2019 Mar 3;9(3):341. doi: 10.3390/nano9030341.
The efficacy of pharmaceutical agents can be greatly improved through nanocarrier delivery. Encapsulation of pharmaceutical agents into a nanocarrier can enhance their bioavailability and biocompatibility, whilst also facilitating targeted drug delivery to specific locations within the body. However, detailed understanding of the in vivo activity of the nanocarrier-drug conjugate is required prior to regulatory approval as a safe and effective treatment strategy. A comprehensive understanding of how nanocarriers travel to, and interact with, the intended target is required in order to optimize the dosing strategy, reduce potential off-target effects, and unwanted toxic effects. Raman spectroscopy has received much interest as a mechanism for label-free, non-invasive imaging of nanocarrier modes of action in vivo. Advanced Raman imaging techniques, including coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), are paving the way for rigorous evaluation of nanocarrier activity at the single-cell level. This review focuses on the development of Raman imaging techniques to study organic nanocarrier delivery in cells and tissues.
通过纳米载体递送可大大提高药剂的疗效。将药剂封装到纳米载体中可提高其生物利用度和生物相容性，同时还便于将药物靶向递送至体内特定位置。然而，在作为一种安全有效的治疗策略获得监管批准之前，需要对纳米载体 - 药物偶联物的体内活性有详细的了解。为了优化给药策略、减少潜在的脱靶效应和不良毒性作用，需要全面了解纳米载体如何到达预期靶点并与之相互作用。拉曼光谱作为一种用于体内纳米载体作用模式的无标记、非侵入性成像机制受到了广泛关注。先进的拉曼成像技术，包括相干反斯托克斯拉曼散射（CARS）和受激拉曼散射（SRS），正在为在单细胞水平上严格评估纳米载体活性铺平道路。本综述重点介绍了用于研究细胞和组织中有机纳米载体递送的拉曼成像技术的发展。
