Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation, Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Henan Xing Rui agriculture and animal husbandry technology Co., LTD, Xinyang 465550, China.
Int J Mol Sci. 2019 Mar 4;20(5):1107. doi: 10.3390/ijms20051107.
Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in adipogenesis and myogenic differentiation, but their impact on porcine muscle traits is not well understood. We compared their expressional profiles in the longissimus dorsi muscle of Chinese Huainan pigs (HN, the fat type) and Western commercial Duroc×(Landrace×Yorkshire) (DLY, the thin type) pigs, and 854 mRNAs, 233 lncRNAs, and 66 circRNAs (p < 0.05 and ｜log₂FoldChange｜>1) were found to be differentially expressed. The differentially expressed mRNA and circRNA parental genes were enriched in the Wnt signaling pathway (adipogenesis), the transition between fast and slow fibers (myogenic differentiation), and alanine, aspartate and glutamate metabolism (pork flavor). The potential lncRNAs/circRNAs-miRNAs-mRNAs regulatory networks shared , , miR-423-5p and miR-874, which were associated with skeletal muscle muscular proliferation, differentiation/regeneration and adipogenesis. Taken together, these differentially expressed non-coding RNAs may be involved in the molecular basis of muscle traits, acting as the competing endogenous RNA (ceRNA) for miRNAs.
环状 RNA（circRNA）和长链非编码 RNA（lncRNA）已知参与脂肪生成和肌生成分化，但它们对猪肌肉性状的影响尚不清楚。我们比较了中国淮南猪（HN，脂肪型）和西方商业杜洛克×（长白猪×约克夏）（DLY，瘦肉型）猪背最长肌中的表达谱，发现 854 个 mRNAs、233 个 lncRNAs 和 66 个 circRNAs（p<0.05 和｜log₂FoldChange｜>1）存在差异表达。差异表达的 mRNA 和 circRNA 亲本基因在 Wnt 信号通路（脂肪生成）、快肌和慢肌纤维之间的转化（肌生成分化）以及丙氨酸、天冬氨酸和谷氨酸代谢（猪肉风味）中富集。共享的潜在 lncRNA/circRNA-miRNA-mRNA 调控网络包括 miR-423-5p 和 miR-874，它们与骨骼肌增殖、分化/再生和脂肪生成有关。综上所述，这些差异表达的非编码 RNA 可能参与肌肉性状的分子基础，作为 miRNA 的竞争性内源 RNA（ceRNA）发挥作用。
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