Nel Sanet, Davis Stephen B, Endo Akihito, Dicks Leon M T
Sugar Milling Research Institute NPC, c/o University of KwaZulu-Natal, Durban, 4041, South Africa.
Department of Microbiology, Stellenbosch University, Stellenbosch, 7600, South Africa.
Curr Microbiol. 2019 May;76(5):527-535. doi: 10.1007/s00284-018-01625-0. Epub 2019 Mar 5.
Polysaccharide (gum)-producing bacteria are responsible for severe economic losses in the sugarcane processing industry. Increased polysaccharide levels in raw sugar are normally an indication that biodeterioration occurred in the cane, soon after harvesting. Once in the sugar processing plant, the cell numbers of gum-producing bacteria escalate and may reach levels difficult to control. We have isolated 430 gum-producing bacteria from sugarcane and different sampling points in a South African sugarcane processing factory. As expected, high cell numbers of gum-producing bacteria were isolated from the factory during a time when sugar with a high dextran content was produced. What we did not expect was to find the same species in the factory at a time when sugar with a low dextran content was produced. Phylogenetic analyses of the 16S rRNA gene sequences differentiated the gum-producing bacteria into four genera and nine species. The majority of these isolates belonged to the genus Weissella (47%), followed by members of Bacillus (24%), Leuconostoc (19%) and Lactobacillus (10%). For the first time, we report on the isolation of Weissella confusa, Weissella cibaria and Bacillus amyloliquefaciens from a sugarcane processing factory.
产生多糖（胶）的细菌给甘蔗加工业造成了严重的经济损失。原糖中多糖水平升高通常表明甘蔗在收获后不久就发生了生物降解。一旦进入制糖厂，产生胶的细菌细胞数量就会激增，可能达到难以控制的水平。我们从南非一家甘蔗加工厂的甘蔗和不同采样点分离出了430株产生胶的细菌。不出所料，在生产含有高葡聚糖含量的糖期间，从工厂中分离出了大量产生胶的细菌。但我们没想到的是，在生产含有低葡聚糖含量的糖时，在工厂中也发现了相同的菌种。对16S rRNA基因序列进行的系统发育分析将产生胶的细菌分为四个属和九个种。这些分离菌株中的大多数属于魏斯氏菌属（47%），其次是芽孢杆菌属成员（24%）、明串珠菌属（19%）和乳杆菌属（10%）。我们首次报道了从甘蔗加工厂分离出了迷惑魏斯氏菌、食窦魏斯氏菌和解淀粉芽孢杆菌。
