Sugar Milling Research Institute NPC, c/o University of KwaZulu-Natal, Durban, 4041, South Africa.
Department of Microbiology, Stellenbosch University, Stellenbosch, 7600, South Africa.
Curr Microbiol. 2019 Oct;76(10):1138-1146. doi: 10.1007/s00284-019-01740-6. Epub 2019 Jul 23.
A previous study reported on the isolation of 430 polysaccharide (gum)-producing bacteria from a South African sugarcane processing factory and the identification of isolates by comparative 16S rRNA gene sequencing. A large number of isolates (202) belonged to the genus Weissella and clustered with reference strains of Weissella cibaria and Weissella confusa. In this study, we identified 147 strains as W. cibaria and 55 as W. confusa based on phylogenetic analyses of pheS and dnaA gene sequences of representative isolates. We also included atpA gene sequence analysis of Weissella isolates as potential future phylogenetic marker to differentiate amongst strains of W. cibaria and W. confusa.
先前的一项研究报告称，从南非的一家甘蔗加工厂中分离出了 430 株多糖（胶）产生菌，并通过比较 16S rRNA 基因测序对分离株进行了鉴定。大量分离株（202 株）属于魏斯氏菌属（Weissella），并与 Weissella cibaria 和 Weissella confusa 的参考菌株聚类。在本研究中，我们根据代表性分离株pheS 和 dnaA 基因序列的系统发育分析，将 147 株鉴定为 W. cibaria，55 株鉴定为 W. confusa。我们还包括 Weissella 分离株的 atpA 基因序列分析，作为区分 W. cibaria 和 W. confusa 菌株的潜在未来系统发育标记。
