Department of Surgery, Faculty of Medicine, Division of Oral Maxillofacial Surgery, Oral Maxillofacial Pathology Unit, Geneva University Hospitals, Geneva, Switzerland.
Department of Oto-Rhino-Laryngology - Head and Neck Surgery, Faculty of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Oral Dis. 2019 Jul;25(5):1283-1290. doi: 10.1111/odi.13084. Epub 2019 Apr 2.
The main objective of this study was to analyze the presence of human papillomavirus (HPV) by polymerase chain reaction (PCR) in invasive squamous cell carcinomas of the oral cavity.
A selection was made of 155 cases of squamous cell carcinoma of the oral cavity treated at the University Hospital of Geneva. HPV detection was performed at the Laboratory of Molecular Pathology using a PCR technique followed by in situ hybridization of the viral DNA. Sections were studied for the immunohistochemical expression of P16 .
The presence of HPV-DNA was found in 3.3% of the cases (95% CI: 1.3%-7.5%). The only HPV genotype found was HR-HPV 16. In contrast, 7.7% (95% CI: 1.3%-7.5%) of the cases showed an overexpression of the P16 . We found no significant differences in age, sex, or tobacco and alcohol consumption in relation to the P16 or HPV positivity.
Human papillomavirus is rarely associated with squamous cell carcinomas of the oral cavity. Our results suggest that an overexpression of the P16 protein, even in the absence of the virus, is linked to an increase in the survival of the patient.
本研究的主要目的是通过聚合酶链反应（PCR）分析口腔浸润性鳞状细胞癌中人类乳头瘤病毒（HPV）的存在。
选择了在日内瓦大学医院治疗的 155 例口腔鳞状细胞癌病例。HPV 检测在分子病理学实验室进行，使用 PCR 技术，随后对病毒 DNA 进行原位杂交。对 P16 的免疫组织化学表达进行了研究。
在 3.3%的病例中发现了 HPV-DNA（95%CI：1.3%-7.5%）。唯一发现的 HPV 基因型是 HR-HPV 16。相比之下，7.7%（95%CI：1.3%-7.5%）的病例显示 P16 过度表达。我们发现 P16 或 HPV 阳性与年龄、性别或烟草和酒精消费之间无显著差异。
人类乳头瘤病毒很少与口腔鳞状细胞癌相关。我们的结果表明，即使在没有病毒的情况下，P16 蛋白的过度表达也与患者生存率的提高有关。
