College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006344.
A Gram-stain-negative bacterium, designated XZ-24, was isolated from sediment of a river in Mianyang city, Sichuan province, PR China. Cells (1.0-2.0 µm long and 0.4-0.5 µm in width) were strictly aerobic, non-spore-forming, rod shaped, prosthecate and motile by means of a polar flagellum. Growth occurred at 10-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum 1.0 % NaCl). The results of phylogenetic analysis based on genomes and 16S rRNA gene sequences indicated that XZ-24 formed a distinct phyletic branch within the family and was most closely related to members of the genera , and with 95.3-96.5 % 16S rRNA gene sequence similarities. The average amino acid identities (AAI) between XZ-24 and species of the family were 47.0-64.5 %, which were below the genus boundary (70 %). The predominant cellular fatty acids were summed feature 8 (Cω7 and/or Cω6), C, Cω7 11-methyl and summed feature 3 (Cω7 and/or Cω6), the isoprenoid quinone was Q-10, and the major polar lipids were 1,2-di-O-acyl-3-O-α-d-glucopyranuronosyl glycerol; 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-α-d glucopyranuronosyl] glycerol and phosphatidylglycerol. The genome size of XZ-24 was 2.64 Mb with a DNA G+C content of 68.9 %. On the basis of the evidence presented in this study, strain XZ-24 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. (Type strain XZ-24=CCTCC AB 20 23 094=KCTC 8038) is proposed.
一株革兰氏阴性细菌，被命名为 XZ-24，从中国四川省绵阳市河流的沉积物中分离得到。细胞（长 1.0-2.0μm，宽 0.4-0.5μm）是严格需氧的、非孢子形成的、杆状、假分枝的和通过极生鞭毛运动。生长发生在 10-37℃（最佳温度 30℃）、pH5.0-9.0（最佳 pH7.0）和 0-3.0%（w/v）NaCl（最佳 1.0%NaCl）。基于基因组和 16S rRNA 基因序列的系统发育分析结果表明，XZ-24 在科内形成了一个独特的进化分支，与属、和的成员最为密切相关，与科内的其他属相比，与属、和的 16S rRNA 基因序列相似性为 95.3-96.5%。XZ-24 与科内其他种的平均氨基酸同一性（AAI）为 47.0-64.5%，低于属的界限（70%）。主要的细胞脂肪酸为总和特征 8（Cω7 和/或 Cω6）、C、Cω7 11-甲基和总和特征 3（Cω7 和/或 Cω6），异戊烯醌为 Q-10，主要的极性脂为 1,2-二酰基-3-O-α-d-吡喃葡萄糖基甘油；1,2-二酰基-3-O-[d-吡喃葡萄糖基-（1→4）-α-d-吡喃葡萄糖基]甘油和磷脂酰甘油。XZ-24 的基因组大小为 2.64Mb，GC 含量为 68.9%。根据本研究提供的证据，菌株 XZ-24 代表了科内一个新属的新种，建议采用属名新属，种名新种（模式菌株 XZ-24=CCTCC AB 2023094=KCTC 8038）。
