Han Hye Seung, Lee Sun-Young, Oh Seo Young, Moon Hee Won, Cho Hyunseok, Kim Ji-Hoon
Department of Pathology, Konkuk University School of Medicine, Seoul 05030, Korea.
Department of Internal Medicine, Konkuk University School of Medicine, Seoul 05030, Korea.
J Clin Med. 2019 Mar 5;8(3):312. doi: 10.3390/jcm8030312.
Mucosal inflammation is characterized by neutrophil and mononuclear cell infiltration. This study aimed to determine the gastric and duodenal microbiota associated with histological, endoscopic, and symptomatic gastritis. Dyspeptic adults who presented for evaluation were included. Subjects with either comorbidities or recent drug intake were excluded. Three endoscopic biopsies were obtained from the antrum, body, and duodenum. Next-generation sequencing for 16S ribosomal RNA V1⁻V2 hypervariable regions was performed. The correlation between the composition of microbiota and the degree of inflammatory cell infiltration, endoscopic findings, and Patient Assessment of Gastrointestinal Disorders Symptom Severity Index (PAGI-SYM) score was analyzed. In 98 included subjects, microbial communities in the antrum and body showed Bray⁻Curtis similarity; however, those in the duodenum showed dissimilarity. Histological and endoscopic gastritis was associated with the abundance of and that of commensal bacteria in the stomach. The abundances of and were correlated with histological gastritis, but not with endoscopic or symptomatic gastritis. The total PAGI-SYM score showed a stronger correlation with the duodenal microbiota ( and ) than with the gastric microbiota (, , and ). Different correlations of the gastric and duodenal microbiota with histological, endoscopic, and symptomatic gastritis were observed for the first time at the species level. -negative gastritis is not associated with endoscopic or symptomatic gastritis. Only -induced endoscopic gastritis requires gastric cancer surveillance. Owing to the weak correlation with , symptomatic gastritis should be assessed separately from histological and endoscopic gastritis.
黏膜炎症的特征是中性粒细胞和单核细胞浸润。本研究旨在确定与组织学、内镜检查及症状性胃炎相关的胃和十二指肠微生物群。纳入前来评估的消化不良成年人。排除患有合并症或近期服用药物的受试者。从胃窦、胃体和十二指肠获取三块内镜活检组织。对16S核糖体RNA V1⁻V2高变区进行二代测序。分析微生物群组成与炎症细胞浸润程度、内镜检查结果以及胃肠道疾病症状严重程度指数患者评估（PAGI-SYM）评分之间的相关性。在98名纳入的受试者中，胃窦和胃体的微生物群落显示出布雷-柯蒂斯相似性；然而，十二指肠的微生物群落显示出差异性。组织学和内镜下胃炎与胃中特定细菌及共生菌的丰度相关。特定细菌的丰度与组织学胃炎相关，但与内镜或症状性胃炎无关。PAGI-SYM总分与十二指肠微生物群（特定细菌）的相关性强于与胃微生物群（特定细菌）的相关性。在物种水平上首次观察到胃和十二指肠微生物群与组织学、内镜及症状性胃炎的不同相关性。特定细菌阴性胃炎与内镜或症状性胃炎无关。仅特定细菌诱导的内镜下胃炎需要进行胃癌监测。由于与特定细菌的相关性较弱，症状性胃炎应与组织学和内镜下胃炎分开评估。
