Yuan Xiang, Liu Yiwen, Kong Jinyu, Gu Bianli, Qi Yijun, Wang Xinshuai, Sun Man, Chen Pan, Sun Wei, Wang Huizhi, Zhou Fuyou, Gao Shegan
Henan Key Laboratory of Cancer Epigenetics, Cancer Hospital, The First Affiliated Hospital, College of Clinical Medicine, Medical College of Henan University of Science and Technology, Luoyang, 471003, China; Department of Medical Oncology, Cancer Hospital, The First Affiliated Hospital, College of Clinical Medicine, Medical College of Henan University of Science and Technology, Luoyang, 471003, China.
Henan Key Laboratory of Cancer Epigenetics, Cancer Hospital, The First Affiliated Hospital, College of Clinical Medicine, Medical College of Henan University of Science and Technology, Luoyang, 471003, China.
Cancer Lett. 2017 Sep 28;404:1-7. doi: 10.1016/j.canlet.2017.07.003. Epub 2017 Jul 11.
The high incidence rate of multiple carcinomas in the upper digestive tract is often explained in terms of involvement of the same underlying risk factors. It has been reported that the oral bacterium Streptococcus anginosus is associated with esophageal, gastric, and pharyngeal cancers. We previously reported occurrence of Porphyromonas gingivalis (P. gingivalis) DNA in esophagus cancer. In this study, the presence of P. gingivalis in specimens of various types of cancer from the upper digestive tract was investigated. Here we report that P. gingivalis was preferentially and frequently present in specimens of esophageal cancer as well as in those from dysplasia of the esophagus but rarely in matched noncancerous portions and are quite low or absent in cancers from the cardia or stomach. Therefore, it led us to propose that, the microorganism does not survive in conditions of high acidity. We then investigate the pH dependence of survival of P. gingivalis as well as the acid tolerance of it. We found that, exposure to acidic buffers of a wide range of pH values led to a decline in colony forming units of P. gingivalis, thus, providing a possible explanation for variations in frequencies of P. gingivalis infection in this study.
上消化道多原发癌的高发病率通常用相同潜在危险因素的影响来解释。据报道，口腔细菌咽峡炎链球菌与食管癌、胃癌和咽喉癌有关。我们之前报道过食管癌中存在牙龈卟啉单胞菌（P. gingivalis）DNA。在本研究中，我们调查了上消化道各种类型癌症标本中牙龈卟啉单胞菌的存在情况。在此我们报告，牙龈卟啉单胞菌优先且频繁地存在于食管癌标本以及食管发育异常标本中，但在匹配的非癌组织中很少见，在贲门癌或胃癌标本中含量很低或不存在。因此，这使我们提出，该微生物在高酸度条件下无法存活。然后我们研究了牙龈卟啉单胞菌存活的pH依赖性及其耐酸性。我们发现，暴露于广泛pH值的酸性缓冲液会导致牙龈卟啉单胞菌的菌落形成单位减少，从而为本研究中牙龈卟啉单胞菌感染频率的差异提供了一种可能的解释。
