Design programme, Indian Institute of Technology, Kanpur, U.P., 208016, India.
Microsystems Fabrication Laboratory, Department of Mechanical Engineering, Indian Institute of Technology, Kanpur, U.P., 208016, India.
Sci Rep. 2019 Mar 6;9(1):3686. doi: 10.1038/s41598-019-40259-5.
In this work we have developed a novel rGO-MWCNT (reduced graphene oxide-multiwalled carbon nanotube) nanocomposite material with Poly-L-Lysine functionalization which can be used for detection of biomolecules with enhanced sensitivity. The reduced GO sheets are found to play a major role as a connector and helps in the assembly of bundles of carbon nanotubes (CNTs) which may sometime play a role of upstanding nanostructures. The overall composite structure is further fully functionalized resulting in an overall high density of amino groups that can be used to capture biomolecules. The sensitivity of the as synthesized film is tested by the oxidation of cholesterol through cholesterol oxidase enzyme that is biochemically immobilized over these composite films. The test for the immobilization density of the novel films are carried out by mounting these films on sensitive thin section static micro/nano-cantilever platforms. The platforms have capability to measure cholesterol traces in blood upto an extent of 100 femto molar through deflection /bending of the cantilevers due to surface reaction. The films developed show a promise of high immobilization density which is further confirmed through fluorescence studies using FITC labeling of functionalized MWCNT-PLL and rGO-PLL films respectively.
在这项工作中，我们开发了一种新型的 rGO-MWCNT（还原氧化石墨烯-多壁碳纳米管）纳米复合材料，具有聚-L-赖氨酸功能化，可用于检测具有增强灵敏度的生物分子。还原的 GO 片被发现主要起连接器的作用，并有助于组装碳纳米管（CNT）束，这些束有时可能起直立纳米结构的作用。整体复合材料结构进一步完全功能化，导致氨基密度整体升高，可用于捕获生物分子。通过将胆固醇氧化酶固定在这些复合膜上，通过胆固醇氧化酶对胆固醇的氧化来测试合成膜的灵敏度。通过将这些膜安装在敏感的薄截面静态微/纳米悬臂平台上来进行新型膜的固定密度测试。这些平台具有通过悬臂的挠度/弯曲来测量血液中胆固醇痕量的能力，最大程度可达 100 飞摩尔，这是由于表面反应所致。所开发的薄膜显示出高固定密度的前景，这通过分别使用 FITC 标记功能化的 MWCNT-PLL 和 rGO-PLL 薄膜进行荧光研究进一步得到证实。
