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二进制 MoS 纳米结构作为纳米载体在多重电化学生物免疫传感中的放大作用：B 细胞激活因子和增殖诱导信号免疫相关细胞因子的同时测定。

Binary MoS nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.

Chronic Disease Programme, UFIEC, Institute of Health Carlos III, 28220, MajadahondaMadrid, Spain.

出版信息

Mikrochim Acta. 2022 Mar 14;189(4):143. doi: 10.1007/s00604-022-05250-4.

DOI:10.1007/s00604-022-05250-4
PMID:35286499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8921152/
Abstract

A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/HO system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.

摘要

本文报道了一种用于同时测定两种重要免疫相关细胞因子的双免疫传感器

BAFF（B 细胞激活因子）和 APRIL（增殖诱导信号）。采用夹心型免疫分析，使用特异性抗体（cAb），并基于用二硫化钼/多壁碳纳米管纳米结构标记检测抗体（dAb）以及使用辣根过氧化物酶（HRP）的信号放大策略。通过氢醌 HQ/HO 体系在丝网印刷双碳电极（SPdCEs）上进行安培检测。所开发的双免疫传感器对 BAFF 和 APRIL 的检测限（LOD）分别为 0.08 和 0.06ng/mL，并且可用于测定癌症细胞裂解物和自身免疫性疾病及癌症患者血清样品中的这两种细胞因子。所得结果与使用 ELISA 方法学得到的结果一致。

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