Braga Vanessa Nogueira Lages, Juanes Camila de Carvalho, Peres Júnior Hélio de Souza, Sousa José Robson de, Cavalcanti Bruno Coêlho, Jamacaru Francisco Vagnaldo Fechine, Lemos Telma Leda Gomes de, Dornelas Conceição Aparecida
Fellow Master degree, Postgraduate Program in Medical Surgical Sciences, School of Medicine, Universidade Federal do Ceará (UFC), Fortaleza-CE, Brazil. Intellectual and scientific content of the study, technical procedures, manuscript preparation.
Fellow Master degree in Pathology, School of Medicine, UFC, Fortaleza-CE, Brazil. Technical procedures, responsible for propolis extraction.
Acta Cir Bras. 2019 Feb 28;34(2):e201900207. doi: 10.1590/s0102-8650201900207.
To evaluate red propolis, gum arabic and L-lysine activity on colorectal preneoplastic lesions induced by azoxymethane (AOM).
The study featured 4 control groups (I-IV) and 4 experimental groups (V-VIII), totaling 48 rats. Once a week for 2 weeks, animals on control groups received saline, while animals in experimental groups received azoxymethane (15 mg/kg i.p.). The follow up along 16 weeks included daily oral gavage to administer water (I and V), L-lysine (150 mg/kg)(II and VI), própolis (100mg/5ml/kg)(III and VII), or gum arabic (5ml/kg)(IV and VIII). Was performed surgery on the animals in the end of this time in order to collect blood for biological assays (TBARS, GSH), followed by their sacrifice to tissue extract.
Oxidative stress (TBARS) and the number of aberrant crypt foci (ACF) in distal colon were lower using própolis (p<0.01 for both parameters). Gum arabic reduced preneoplastic lesions (ACF ≤ 4 crypts) on distal colon and on the entire colon (p<0.05).
Red propolis reduced AOM-induced oxidative stress (TBARS) and total number of ACF in the distal colon. L-lysine neither protected against nor enhanced AOM-induced ACF. Gum arabic reduced the number of ACF.
评估红蜂胶、阿拉伯胶和L-赖氨酸对由氧化偶氮甲烷（AOM）诱导的结直肠肿瘤前病变的作用。
该研究设有4个对照组（I-IV）和4个实验组（V-VIII），共计48只大鼠。对照组动物每周一次，持续2周，接受生理盐水，而实验组动物接受氧化偶氮甲烷（15mg/kg腹腔注射）。16周的随访包括每日经口灌胃给予水（I和V）、L-赖氨酸（150mg/kg）（II和VI）、蜂胶（100mg/5ml/kg）（III和VII）或阿拉伯胶（5ml/kg）（IV和VIII）。在这段时间结束时对动物进行手术，以采集血液进行生物学检测（硫代巴比妥酸反应物、谷胱甘肽），随后将它们处死以获取组织提取物。
使用蜂胶时，远端结肠的氧化应激（硫代巴比妥酸反应物）和异常隐窝灶（ACF）数量较低（两个参数均p<0.01）。阿拉伯胶减少了远端结肠和整个结肠的肿瘤前病变（ACF≤4个隐窝）（p<0.05）。
红蜂胶降低了AOM诱导的氧化应激（硫代巴比妥酸反应物）和远端结肠ACF的总数。L-赖氨酸既未预防也未增强AOM诱导的ACF。阿拉伯胶减少了ACF的数量。
