Filicori M, Santoro N, Merriam G R, Crowley W F
J Clin Endocrinol Metab. 1986 Jun;62(6):1136-44. doi: 10.1210/jcem-62-6-1136.
To characterize the spectrum of pulsatile gonadotropin secretion during the course of the normal menstrual cycle, we studied normal women during 51 ovulatory cycles. Plasma gonadotropin concentrations were measured at 10-min intervals for 20-24 h during the early, mid-, and late follicular phases and the early, mid-, and late luteal phases. LH data series were analyzed using 2 different computer-assisted algorithms for pulse detection. The LH interpulse interval decreased during the follicular phase (FP) from 94 +/- 4 (+/- SEM) min in the early FP (EFP) to 71 +/- 4 min by the late FP (LFP; P less than 0.001). The estimation of LH pulse frequency in the EFP was significantly affected by slowing of episodic LH secretion during sleep. In the luteal phase (LP), the LH interpulse interval progressively increased from 103 +/- 8 min in the early LP (ELP) to 216 +/- 39 min by the late LP (LLP; P less than 0.001). Sleep-associated slowing of episodic LH secretion also occurred in the ELP. The mean LH pulse amplitude in the EFP (6.5 +/- 0.4 mIU/ml) decreased significantly by the midfollicular phase (MFP; 5.1 +/- 0.8 mIU/ml; P less than 0.05) and increased once again by the LFP (7.2 +/- 1.2 mIU/ml). LH pulse amplitude was highest in the ELP (14.9 +/- 1.7 mIU/ml), decreased by the midluteal phase (MLP) to 12.2 +/- 2.0 mIU/ml, and declined further by the LLP to 7.6 +/- 1.1 mIU/ml (P less than 0.001 vs. ELP). FSH secretion was significantly (P less than 0.05) correlated with LH secretion at time lags of 0-10 min in 82% of the studies. These results indicate the following. 1) In the EFP and ELP, the frequency of gonadotropin pulsations is reduced at night in association with sleep. 2) The frequency of LH secretion increases from the EFP to MFP and LFP. 3) LH pulse amplitude decreases in the MFP, suggesting enhanced negative feedback of estrogen on the hypothalamic-pituitary axis and/or a decrease in GnRH secretion at this stage. 4) A progressive reduction of LH pulse frequency and amplitude occurs during the LP which is correlated with the duration of exposure of the hypothalamic-pituitary axis to progesterone. 5) A close relationship exists between secretion of LH and FSH, suggesting a common stimulatory factor for both gonadotropins.
为了描述正常月经周期中促性腺激素脉冲式分泌的特征，我们对51个排卵周期的正常女性进行了研究。在卵泡期早期、中期和晚期以及黄体期早期、中期和晚期，每隔10分钟测量一次血浆促性腺激素浓度，持续20 - 24小时。使用2种不同的计算机辅助算法分析LH数据系列以检测脉冲。卵泡期（FP）期间，LH脉冲间期从卵泡期早期（EFP）的94±4（±SEM）分钟降至卵泡期晚期（LFP）的71±4分钟（P<0.001）。睡眠期间LH分泌的减慢显著影响了EFP中LH脉冲频率的估计。在黄体期（LP），LH脉冲间期从黄体期早期（ELP）的103±8分钟逐渐增加至黄体期晚期（LLP）的216±39分钟（P<0.001）。ELP中也出现了与睡眠相关的LH分泌减慢。EFP中的平均LH脉冲幅度（6.5±0.4 mIU/ml）在卵泡中期（MFP）显著下降（5.1±0.8 mIU/ml；P<0.05），并在LFP时再次升高（7.2±1.2 mIU/ml）。LH脉冲幅度在ELP中最高（14.9±1.7 mIU/ml），在黄体中期（MLP）降至12.2±2.0 mIU/ml，并在LLP时进一步降至7.6±1.1 mIU/ml（与ELP相比，P<{0.001}）。在82%的研究中，FSH分泌与LH分泌在0 - 10分钟的时间滞后显著相关（P<0.05）。这些结果表明：1）在EFP和ELP中，夜间与睡眠相关的促性腺激素脉冲频率降低。2）LH分泌频率从EFP增加到MFP和LFP。3）MFP中LH脉冲幅度降低，表明雌激素对下丘脑 - 垂体轴的负反馈增强和/或此阶段GnRH分泌减少。4）LP期间LH脉冲频率和幅度逐渐降低，这与下丘脑 - 垂体轴暴露于孕酮的持续时间相关。5）LH和FSH分泌之间存在密切关系，表明两种促性腺激素有共同的刺激因子。
