Frøholm L O, Bøvre K, Holten E, Zollinger W D
NIPH Ann. 1983 Dec;6(2):125-31.
Treatment of agar-grown meningococci with dilute hydrochloric acid and suspension in saline after washing gave a preparation suitable for meningococcal serotyping by staphylococcal coagglutination with monoclonal antibodies. The monoclonal antibodies available for use and attached to protein A on the staphylococci were directed against the serotype protein antigens 2a, 2b (class 2), 15 (class 3) and the subtype protein antigens P1. 2 and P1. 16 (class 1). Ninety per cent of systemic strains and thirty-four per cent of a collection of carrier isolates, both from Norway late 1981/early 1982, were typable. The serotype antigen 15 alone or in combination with P1. 16 or P1. 16 alone were detected in about 85 per cent of the systemic strains. The quality of the whole-cell meningococcal antigen was important for the test to be easily read.
用稀盐酸处理琼脂培养的脑膜炎球菌，洗涤后悬浮于盐水中，得到一种适合通过与单克隆抗体进行葡萄球菌协同凝集来进行脑膜炎球菌血清分型的制剂。可用于并附着在葡萄球菌蛋白A上的单克隆抗体针对血清型蛋白抗原2a、2b（2类）、15（3类）以及亚型蛋白抗原P1.2和P1.16（1类）。来自1981年末/1982年初挪威的90%的系统性菌株和34%的携带者分离株集合可进行分型。单独的血清型抗原15或与P1.16组合或单独的P1.16在约85%的系统性菌株中被检测到。全细胞脑膜炎球菌抗原的质量对于易于读取检测结果很重要。
