狂犬病病毒自然感染：人脑的组织病理学和免疫组织化学研究
Natural Infection with Rabies Virus: A Histopathological and Immunohistochemical Study of Human Brains.
作者信息
Farahtaj Firouzeh, Alizadeh Leila, Gholami Alireza, Tahamtan Alireza, Shirian Sadegh, Fazeli Maryam, Nejad Amir Sasan Mozaffari, Gorji Ali, Niknam Hamid Mahmoudzadeh, Ghaemi Amir
机构信息
Collaborating Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran.
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
出版信息
Osong Public Health Res Perspect. 2019 Feb;10(1):6-11. doi: 10.24171/j.phrp.2019.10.1.03.
OBJECTIVES
Despite all the efforts and increased knowledge of rabies, the exact mechanisms of infection and mortality from the rabies virus are not well understood. To understand the mechanisms underlying the pathogenicity of rabies virus infection, it is crucial to study the tissue that the rabies virus naturally infects in humans.
METHODS
Cerebellum brain tissue from 9 human post mortem cases from Iran, who had been infected with rabies virus, were examined histopathologically and immunohistochemically to evaluate the innate immune responses against the rabies virus.
RESULTS
Histopathological examination revealed inflammation of the infected cerebellum and immunohistochemical analyses showed an increased immunoreactivity of heat shock protein 70, interleukin-6, interleukin-1, tumor necrosis factor-alpha, caspase-3, caspase-9, toll-like receptor3 and toll-like receptor4 in the infected brain tissue.
CONCLUSION
These results indicated the involvement of innate immunity in rabies infected human brain tissue, which may aggravate the progression of this deadly disease.
目的
尽管在狂犬病研究方面付出了诸多努力且知识不断增加，但狂犬病病毒感染和致死的确切机制仍未完全明晰。为了解狂犬病病毒感染致病性的潜在机制，研究狂犬病病毒在人类自然感染的组织至关重要。
方法
对来自伊朗的9例狂犬病病毒感染死亡病例的小脑脑组织进行组织病理学和免疫组织化学检查，以评估针对狂犬病病毒的先天免疫反应。
结果
组织病理学检查显示受感染的小脑出现炎症，免疫组织化学分析表明，受感染脑组织中热休克蛋白70、白细胞介素-6、白细胞介素-1、肿瘤坏死因子-α、半胱天冬酶-3、半胱天冬酶-9、Toll样受体3和Toll样受体4的免疫反应性增加。
结论
这些结果表明先天免疫参与了狂犬病感染的人类脑组织，这可能会加剧这种致命疾病的进展。
Zotero 插件