Arumugam T, Jayapriya G, Sekar T
Biotech Research and Development, Bioplus Life Science, Hosur, 635109, Tamil Nadu, India.
Department of Chemistry, Government Arts and Science College, Dharmapuri, 636701, Tamil Nadu, India.
Biotechnol Rep (Amst). 2019 Feb 16;21:e00318. doi: 10.1016/j.btre.2019.e00318. eCollection 2019 Mar.
Genomic DNA of (Dennst) of sixteen geographical regions were amplified using thirteen primers. The analyses of products of Radom Amplified Polymorphic DNA (RAPD) revealed polymorphism among the samples. The polymorphic information content (PIC) was maximum for the primer of OPB-04 (0.40) followed by the primer OPB-20 (0.39). Clustering analysis by Jaccard's coefficient index exhibited similarity matrix and distance matrix. The amplified products of the 13 primers collectively showed similarity index ranged from 0.654 to 0.986. The phylogenetic trees were constructed by UPGMA cluster analysis. Significant variations in the amplified genomic DNA suggest genetic variability of population but low inter-population genetic segregation.
使用13种引物对16个地理区域的（某种植物，原文未明确写出具体植物名，推测是某种蕨类植物 Dennst）基因组DNA进行扩增。随机扩增多态性DNA（RAPD）产物分析显示样本间存在多态性。引物OPB - 04的多态信息含量（PIC）最高（0.40），其次是引物OPB - 20（0.39）。通过杰卡德系数指数进行聚类分析，展示了相似性矩阵和距离矩阵。13种引物的扩增产物共同显示相似性指数范围为0.654至0.986。通过UPGMA聚类分析构建了系统发育树。扩增的基因组DNA中的显著变异表明种群具有遗传变异性，但种群间的遗传分化较低。
