Hokkaido Research Organization (HRO) Central Agricultural Experiment Station (AES) , Higashi 6 Kita 15, Naganuma, Hokkaido 069-1395 , Japan.
HRO Kitami AES , 52 Yayoi, Kunneppu, Hokkaido 099-1496 , Japan.
Breed Sci. 2014 Dec;64(4):404-8. doi: 10.1270/jsbbs.64.404. Epub 2014 Dec 1.
The yellowing strain of Soybean dwarf virus (SbDV-YS) causes yellowing and yield loss in common bean (Phaseolus vulgaris). The most effective control is achieved through breeding for resistance. An indeterminate climbing cultivar with a white seed coat, 'Oofuku', is resistant to SbDV-YS in inoculation tests. We crossed 'Oofuku' with an elite cultivar, 'Taisho-Kintoki', which is SbDV-YS-susceptible, determinate dwarf with a red-purple seed coat, and performed amplified-fragment-length polymorphism analysis of F3 lines. From nucleotide sequences of the resistant-specific fragments and their flanking regions, we developed five DNA markers, of which DV86, DV386, and DV398 were closely linked to Sdvy-1, a resistance gene. Using the markers, we developed 'Toiku-B79' and 'Toiku-B80', the near-isogenic lines (NILs) incorporating Sdvy-1 in the background of 'Taisho-Kintoki'. The NILs had similar growth habit, maturity date and seed shape to those of 'Taisho-Kintoki'. The quality of boiled beans was also similar, except that the NILs had more seed coat cracking than 'Taisho-Kintoki'. The NILs showed no SbDV-YS infection in inoculation tests. We suggest that Sdvy-1 is a useful source of SbDV-YS resistance in common bean.
黄化矮缩病毒（SbDV-YS）的黄化株系可导致菜豆（Phaseolus vulgaris）黄化和减产。最有效的防治方法是通过抗性育种。一种不定根攀援的白色种皮品种“大幸”在接种试验中对 SbDV-YS 具有抗性。我们将“大幸”与易感 SbDV-YS 的、确定矮化的、红色-紫色种皮的优良品种“Taisho-Kintoki”进行杂交，并对 F3 系进行扩增片段长度多态性分析。从抗性特异片段及其侧翼区域的核苷酸序列中，我们开发了 5 个 DNA 标记，其中 DV86、DV386 和 DV398 与 Sdvy-1 紧密连锁，Sdvy-1 是一个抗性基因。利用这些标记，我们开发了“Toiku-B79”和“Toiku-B80”，这两个近等基因系（NILs）在“Taisho-Kintoki”的背景下含有 Sdvy-1。NILs 的生长习性、成熟期和种子形状与“Taisho-Kintoki”相似。煮豆的品质也相似，只是 NILs 的种皮开裂比“Taisho-Kintoki”多。在接种试验中，NILs 未感染 SbDV-YS。我们认为 Sdvy-1 是菜豆 SbDV-YS 抗性的有用来源。
