Manickam Arun, Singh Rituraj, McDermott Mark, Wood Nicholas, Bolouki Sara, Naraghi-Arani Pejman, Johnson Kirsten, Kuimelis Robert G, Schoolnik Gary, Hassibi Arjang
InSilixa, Inc., 1000 Hamlin Ct., Sunnyvale, CA 94089, United States.
IEEE J Solid-State Circuits. 2017 Nov;52(11):2857-2870. doi: 10.1109/JSSC.2017.2754363. Epub 2017 Oct 24.
Design and successful implementation of a fully-integrated CMOS fluorescence biochip for DNA/RNA testing in molecular diagnostics (MDx) is presented. The biochip includes a 32×32 array of continuous wave fluorescence detection biosensing elements. Each biosensing element is capable of having unique DNA probe sequences, wavelength-selective multi-dielectric emission filter (OD of 3.6), resistive heater for thermal cycling, and a high performance and programmable photodetector. The dimension of each biosensor is 100m×100m with a 50m×50m N-P photodiode acting as the optical transducer, and a ΣΔ modulator based photocurrent sensor. The measured photodetector performance shows ~116 dB detection dynamic range (10fA - 10nA) over the 25°C - 100°C temperature range, while being ~1 dB away from the fundamental shot-noise limit. To empirically demonstrate the compatibility of this biochip with MDx applications, we have successfully utilized the array and its thermal cycling capability to adopt a 7-plex panel for detection of 6 human upper respiratory viruses.
本文介绍了一种用于分子诊断（MDx）中DNA/RNA检测的全集成CMOS荧光生物芯片的设计与成功实现。该生物芯片包括一个32×32的连续波荧光检测生物传感元件阵列。每个生物传感元件能够具有独特的DNA探针序列、波长选择性多介质发射滤光片（光密度为3.6）、用于热循环的电阻加热器以及高性能可编程光电探测器。每个生物传感器的尺寸为100μm×100μm，其中一个50μm×50μm的N-P光电二极管用作光传感器，以及一个基于ΣΔ调制器的光电流传感器。所测得的光电探测器性能显示，在25°C至100°C的温度范围内，检测动态范围约为116 dB（10fA - 10nA），同时与基本散粒噪声极限相差约1 dB。为了通过实验证明该生物芯片与MDx应用的兼容性，我们成功利用该阵列及其热循环能力采用了一个7重检测面板来检测6种人类上呼吸道病毒。
