Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.
Central animal lab facility, Amrita Institute of Medical sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.
Nanomedicine. 2019 Jun;18:179-188. doi: 10.1016/j.nano.2019.02.022. Epub 2019 Mar 8.
We report an osteoconducting magnetic 3D scaffold using Fe doped nano-hydroxyapatite-Alginate-Gelatin (AGHFe1) for Magnetic Resonance Imaging based non-invasive monitoring of bone tissue regeneration. In rat cranial defect model, the scaffold facilitated non-invasive monitoring of cell migration, inflammatory response and matrix deposition by unique changes in transverse relaxation time (T2). Cell infiltration resulted in a considerable increase in T2 from 37 to ~62 ms, which gradually returned to that of native bone (23 ms) by 90 days. We used this method to compare in vivo performance of scaffold with bone-morphogenic protein-2 (AGHFe2) or faster degrading (AGHFe3). MRI and histological analysis over 90 days showed non-uniform bone formation in AGHFe1 with ∆T2 (T2 - T2 ) ~13 ms, whereas, AGHFe2 and AGHFe3 showed ∆T2 ~ 09 and 05 ms respectively, suggesting better bone formation in AGHFe3. Thus, we show that MR-contrast enabled scaffold can help better assessment of bone-regeneration non-invasively.
我们报告了一种使用 Fe 掺杂纳米羟基磷灰石-藻酸盐-明胶（AGHFe1）的骨诱导性磁性 3D 支架，用于基于磁共振成像的骨组织再生的非侵入性监测。在大鼠颅缺损模型中，支架通过横向弛豫时间（T2）的独特变化促进细胞迁移、炎症反应和基质沉积的非侵入性监测。细胞浸润导致 T2 从约 37 毫秒显著增加到约 62 毫秒，在 90 天内逐渐恢复到天然骨（约 23 毫秒）。我们使用这种方法比较了支架与骨形态发生蛋白-2（AGHFe2）或更快降解（AGHFe3）的体内性能。90 天的 MRI 和组织学分析显示，AGHFe1 中的骨形成不均匀，∆T2（T2-T2）约为 13 毫秒，而 AGHFe2 和 AGHFe3 的∆T2 分别约为 09 和 05 毫秒，表明 AGHFe3 中的骨形成更好。因此，我们表明磁共振对比增强支架可以帮助更好地非侵入性评估骨再生。
