Forestry and Forest Products Research Institute, Forest Research and Management Organization, Matsunosato, Tsukuba, Ibaraki 305-8687, Japan.
J Invertebr Pathol. 2020 Jul;174:107417. doi: 10.1016/j.jip.2020.107417. Epub 2020 Jun 1.
A cypovirus was isolated from larvae of the Japanese peppered moth, Biston robustus. The viral genome is 23,954 bp comprising 10 segmented double-stranded RNAs with a new electropherotype among cypoviruses. Each segment encodes one putative protein and has non-coding regions that contain conserved sequences at their 5' and 3' termini, 5'-AGAA(U/A)U-3' and 5'-UGC-3', respectively. Seven proteins encoded in the genome are homologous to those of other cypoviruses at a cut-off E-value of 1 × 10. The maximal sequence identities of these proteins with cypovirus homologs are 24.30%-39.40%. These results indicate that the virus isolated is a novel cypovirus; herein designated as Biston robustus cypovirus 24 (BrCPV-24). This newly isolated BrCPV-24 infects the larvae of the silkworm Bombyx mori.
从日本丽叩甲幼虫中分离到一株细胞多角体病毒。该病毒基因组全长 23954bp，由 10 个分段的双链 RNA 组成，在细胞多角体病毒中具有一种新的电泳图谱类型。每个片段编码一个假定的蛋白质，并且在其 5' 和 3' 末端具有非编码区，分别为 5'-AGAA（U/A）U-3' 和 5'-UGC-3'。基因组中编码的 7 种蛋白质与其他细胞多角体病毒的蛋白质在截止 E 值为 1×10-10 时有同源性。这些蛋白质与细胞多角体病毒同源物的最大序列同一性为 24.30%-39.40%。这些结果表明，分离到的病毒是一种新型的细胞多角体病毒；在此将其命名为日本丽叩甲细胞多角体病毒 24（BrCPV-24）。新分离的 BrCPV-24 可感染家蚕幼虫。
