Ploidy Determination in the Pathogenic Fungus spp.

The pathogenic clade of the genus comprises the etiological agents of sporotrichosis, a worldwide emergent disease. Despite the growing understanding of their successful pathogen traits, there is little information on genome sizes and ploidy within the genus. Therefore, in this work, we evaluated the ploidy of four species of the genus, specifically , , , and . Through cell cycle analysis of the yeast-phase cells, we showed that the DNA content of G/G cells was similar to the genome size determined by whole genome sequencing. Moreover, ploidy of , , and that was determined by allele composition using next-generation sequencing (NGS) data is consistent with monomorphic positions at each allele. These data show that the analyzed strains of are haploid, or at least aneuploid, thereby laying the foundation for the development of a molecular toolbox for spp.