ICFO-Institut de Ciències Fotòniques , The Barcelona Institute of Science and Tehcnology , 08860 Castelldefels (Barcelona) , Spain.
Department of Research and Development , B. Braun Surgical, S.A. , 08191 Rubí, Barcelona , Spain.
Nano Lett. 2019 Apr 10;19(4):2524-2529. doi: 10.1021/acs.nanolett.9b00187. Epub 2019 Mar 18.
The insertion of an implant in the body of a patient raises the risk of a posterior infection and formation of a biofilm, which can have critical consequences on the patient's health and be associated with a high sanitary cost. While antibacterial agents can be used to prevent the infection, such a strategy is time-limited and causes bacteria resistance. As an alternative to biochemical approaches, we propose here to use light-induced local hyperthermia with plasmonic nanoparticles. This strategy is implemented on surgical meshes, extensively used in the context of hernia repairing, one of the most common general surgeries. Surgical meshes were homogeneously coated with gold nanorods designed to efficiently convert near-infrared light into heat. The modified mesh was exposed to a biofilm of Staphylococcus aureus ( S. aureus) bacteria before being treated with a train of light pulses. We systematically study how the illumination parameters, namely fluence, peak intensity and pulse length, influence the elimination of attached bacteria. Additionally, fluorescence confocal microscopy provides us some insight on the mechanism involved in the degradation of the biofilm. This proof-of-principle study opens a new set of opportunities for the development of novel disinfection approaches combining light and nanotechnology.
将植入物插入患者体内会增加后部感染和生物膜形成的风险，这可能对患者的健康产生严重后果，并与高卫生成本有关。虽然可以使用抗菌剂来预防感染，但这种策略是有限的，并会导致细菌耐药性。作为生化方法的替代方案，我们在这里提出使用等离子体纳米粒子的光诱导局部热疗。该策略应用于手术网片上，手术网片广泛用于疝修补术，疝修补术是最常见的普通外科手术之一。金纳米棒均匀地涂覆在手术网片上，金纳米棒可以有效地将近红外光转化为热量。在使用一系列光脉冲进行处理之前，用金黄色葡萄球菌 (S. aureus) 细菌的生物膜对修饰后的网片进行处理。我们系统地研究了照射参数（即剂量、峰值强度和脉冲长度）如何影响附着细菌的消除。此外，荧光共焦显微镜使我们对生物膜降解过程中涉及的机制有了一些了解。这项原理验证研究为结合光和纳米技术开发新型消毒方法开辟了一系列新的机会。
