新兴的橡皮擦酶在蛋白质 ADP-核糖基化的动态调控中的作用。

Genome Stability Laboratory, Centre de Recherche du Centre Hospitalier Universitaire de Québec-Université Laval, HDQ Pavilion, Oncology Division, Québec, G1R 2J6, Canada.

Département de Biologie Moléculaire, Biochimie Médicale et Pathologie, Faculté de Médecine, Université Laval, Québec, G1V 0A6, Canada.

Nat Commun. 2019 Mar 12;10(1):1182. doi: 10.1038/s41467-019-08859-x.

Protein ADP-ribosylation is essential for the regulation of several cellular pathways, enabling dynamic responses to diverse pathophysiological conditions. It is modulated through a dynamic interplay between ADP-ribose readers, writers and erasers. While ADP-ribose synthesis has been studied and reviewed extensively, ADP-ribose processing by erasing enzymes has received comparably less attention. However, major progress in the mass spectrometric identification of ADP-ribosylated residues and the biochemical characterization of ADP-ribose erasers has substantially expanded our knowledge of ADP-ribosylation dynamics. Herein, we describe recent insights into the biology of ADP-ribose erasers and discuss the intricately orchestrated cellular processes to switch off ADP-ribose-dependent mechanisms.

蛋白 ADP-核糖基化对于调节多种细胞途径至关重要，使细胞能够对各种病理生理条件做出动态响应。它通过 ADP-核糖基读码器、写码器和消除酶之间的动态相互作用来调节。虽然 ADP-核糖基的合成已经得到了广泛的研究和综述，但 ADP-核糖基的消除酶对其的处理却受到了相对较少的关注。然而，质谱鉴定 ADP-核糖基化残基和生化表征 ADP-核糖基消除酶方面的重大进展，极大地扩展了我们对 ADP-核糖基化动力学的认识。本文描述了 ADP-核糖基消除酶生物学的最新见解，并讨论了精细协调的细胞过程，以关闭 ADP-核糖基依赖性机制。