LBCMCP, Centre de Biologie Intégrative, CNRS, Toulouse, France.
Université Toulouse III: Paul-Sabatier, Toulouse, France.
Sci Rep. 2019 Mar 12;9(1):4181. doi: 10.1038/s41598-019-40873-3.
Discovery of protein modification sites relies on protein digestion by proteases and mass spectrometry (MS) identification of the modified peptides. Depending on proteases used and target protein sequence, this method yields highly variable coverage of modification sites. We introduce PTMselect, a digestion-simulating software which tailors the optimal set of proteases for discovery of global or targeted modification from any single or multiple proteins.
蛋白质修饰位点的发现依赖于蛋白酶对蛋白质的消化和质谱（MS）对修饰肽的鉴定。根据所使用的蛋白酶和目标蛋白质序列，该方法对修饰位点的覆盖度变化很大。我们引入了 PTMselect，这是一种消化模拟软件，可以针对任何单个或多个蛋白质，为全局或靶向修饰的发现定制最佳蛋白酶集。
