Hewinson R G, Lane D C, Slack M P, Nichols W W
J Gen Microbiol. 1986 Jan;132(1):27-33. doi: 10.1099/00221287-132-1-27.
The permeability parameter (C) for the movement of cephalosporin C across the outer membrane of Pseudomonas aeruginosa was measured using the widely accepted method of Zimmermann & Rosselet. In one experiment, the value of C varied continuously from 4.2 to 10.8 cm3 min-1 (mg dry wt cells)-1 over a range of concentrations of the test substrate, cephalosporin C, from 50 to 5 microM. Dependence of C on the concentration of test substrate was still observed when the effect of a possible electric potential difference across the outer membrane was corrected for. In quantitative studies of beta-lactam permeation the dependence of C on the concentration of beta-lactam should be taken into account.
使用广泛认可的齐默尔曼和罗塞莱特方法，测定了头孢菌素C穿过铜绿假单胞菌外膜的渗透参数（C）。在一项实验中，在测试底物头孢菌素C的浓度范围从50到5微摩尔时，C值在4.2至10.8立方厘米每分钟每毫克干重细胞的范围内持续变化。当校正了跨外膜可能存在的电势差的影响后，仍观察到C对测试底物浓度的依赖性。在β-内酰胺渗透的定量研究中，应考虑C对β-内酰胺浓度的依赖性。
