College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
Int J Mol Sci. 2020 Jan 17;21(2):608. doi: 10.3390/ijms21020608.
Heat shock transcription factors (HSFs) play crucial roles in resisting heat stress and regulating plant development. Recently, HSFs have been shown to play roles in anther development. Thus, investigating the HSF family members and identifying their protective roles in anthers are essential for the further development of male sterile wheat breeding. In the present study, 61 wheat HSF genes () were identified in the whole wheat genome and they are unequally distributed on 21 chromosomes. According to gene structure and phylogenetic analyses, the 61 s were classified into three categories and 12 subclasses. Genome-wide duplication was identified as the main source of the expansion of the wheat HSF gene family based on 14 pairs of homeologous triplets, whereas only a very small number of were derived by segmental duplication and tandem duplication. Heat shock protein 90 (HSP90), HSP70, and another class of chaperone protein called htpG were identified as proteins that interact with wheat HSFs. RNA-seq analysis indicated that have obvious period- and tissue-specific expression patterns, and the in classes A and B respond to heat shock, whereas the C class are involved in drought regulation. qRT-PCR identified three with differential expression in sterile and fertile anthers, and they may be candidate genes involved in anther development. This comprehensive analysis provides novel insights into , and it will be useful for understanding the mechanism of plant fertility conversion.
热休克转录因子（HSFs）在抵抗热应激和调节植物发育中发挥着重要作用。最近，HSFs 被证明在花药发育中起作用。因此，研究 HSF 家族成员并鉴定它们在花药中的保护作用，对于进一步发展小麦雄性不育系的选育是至关重要的。本研究在整个小麦基因组中鉴定了 61 个小麦 HSF 基因（），它们在 21 条染色体上不均匀分布。根据基因结构和系统发育分析，将 61 个分为三类和 12 个亚类。基于 14 对同源三核苷酸对，全基因组复制被认为是小麦 HSF 基因家族扩张的主要来源，而仅有极少数的通过片段复制和串联复制产生。热休克蛋白 90（HSP90）、HSP70 和另一类称为 htpG 的伴侣蛋白被鉴定为与小麦 HSFs 相互作用的蛋白。RNA-seq 分析表明，有明显的周期和组织特异性表达模式，A 类和 B 类的在热应激下响应，而 C 类则参与干旱调节。qRT-PCR 鉴定了三个在不育和可育花药中表达差异的，它们可能是参与花药发育的候选基因。这项综合分析为 HSF 提供了新的见解，将有助于理解植物育性转换的机制。
